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Digitale Medien

Differential location of regulatory and nonregulatory trehalases inCandida utilis cells (1988)

Arguelles, J. C. ; Gacto, M.

Springer

Antonie van Leeuwenhoek 54 (1988), S. 555-565

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Details

ISSN: 1572-9699

Schlagwort(e): trehalases ; vacuoles ; glycoproteins ; Candida utilis

Quelle: Springer Online Journal Archives 1860-2000

Thema: Biologie

Notizen: Abstract The isolation of vacuoles by density gradient centrifugation of protoplast lysates fromCandida utilis cells showed a high specific activity for nonregulatory trehalase in vacuoles whereas the regulatory trehalase activatable by phosphorylation behaves as a cytoplasmic enzyme. The vacuolar trehalase is a glycoprotein that can be precipitated by Con A-Sepharose. Treatment of this enzyme with endo H reduced its reactivity with the lectin without loss of enzyme activity and decreased its apparent molecular weight by gel filtration.

Materialart: Digitale Medien

URL: http://dx.doi.org/10.1007/BF00588391

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