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  • Articles: DFG German National Licenses  (16)
  • Electronic Resource  (16)
  • 2005-2009
  • 2000-2004  (16)
  • 2004  (16)
Source
  • Articles: DFG German National Licenses  (16)
Material
  • Electronic Resource  (16)
Years
  • 2005-2009
  • 2000-2004  (16)
Year
  • 1
    Electronic Resource
    Electronic Resource
    005
Cyclodextrin Polymer Based Biocompatible Matrix for Local Gene Delivery (2004)
    Oxford, UK; Malden, USA : Blackwell Science Ltd/Inc.
    Wound repair and regeneration 12 (2004), S. 0 
    Details
    ISSN: 1524-475X
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: Biocompatible matrices, such as bovine collagen, have demonstrated usefulness in delivering gene therapy vectors that express growth factors to local environments for tissue repair. Unlike animal derived matrices, we have developed a new synthetic matrix consisting of a linear cyclodextrin-polyethyleneglycol co-polymer that is non-covalently cross-linked with di-adamantane-polyethyleneglycol via inclusion complex formation between adamantane and cyclodextrin (CD-Ada). We performed both in vitro and in vivo experiments for biocompatibility and localized transgene expression using a recombinant adenovirus (rAd) vector containing either the reporter gene, GFP, or the therapeutic gene, PDGF-B. In vitro results demonstrated cell migration, adenoviral transduction, and gene expression with no visible signs of toxicity in human skin fibroblasts. Qualitative gene expression from the CD-Ada containing rAd was delayed by approximately two days when compared to collagen, but the level of expression was greater over a longer period of time. In vivo experiments demonstrated gene expression after local delivery to mouse skin using rAd-GFP in CD-Ada. Again, the expression was slightly delayed but duration of expression was comparable to collagen. Expression studies using rAd-PDGF-B, were performed in the rat polyvinyl alcohol sponge model and showed comparable quantitative DNA and RNA levels between CD-Ada and collagen (DNA: 4.1 × 1010 and 4.5 × 1010 MEQ of PDGF-B/assayed sponge, respectively; RNA: 7.0 × 108 and 3.2 × 108 MEQ of PDGF-B/assayed sponge, respectively). Additionally, we explored the use of plasmid DNA with the CD-Ada matrix and observed PDGF-B expression in vivo. Our results show that this new delivery system provides a safe, efficient, and adaptable medium for both viral and non-viral gene delivery.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1067-1927.2004.0abstractf.x
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  • 2
    Electronic Resource
    Electronic Resource
    036
Overexpression of Smad7 in Keratinocytes Accelerates Cutaneous Wound Healing (2004)
    Oxford, UK; Malden, USA : Blackwell Science Ltd/Inc.
    Wound repair and regeneration 12 (2004), S. 0 
    Details
    ISSN: 1524-475X
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: Transforming growth factor β(TGFβ) has both positive and negative effects on cutaneous wound healing. Smad7 acts as a major downstream antagonist of TGFβ signaling in keratinocytes and its role in wound healing has not been defined. We have established a Smad7 transgenic mouse line using a keratin 5 (K5) promoter (K5.Smad7) which expresses Smad7 transgene at a mild level (∼2 fold of the endogenous Smad7 in the skin). These mice did not have overt skin defects as shown from our previous Smad7 transgenic mice expressing much higher levels of the Smad7 transgene (EMBO J 2002, 21:2580–90). K5.Smad7 mice from the above low expressor line and non-transgenic littermates were subject to 6-mm full-thickness excisional wounding. K5.Smad7 mice exhibited early scab rejection, reduced inflammation, and accelerated re-epithelialization as compared to non-transgenic mice. To further determine the stage-specific effects of Smad7 on wound healing, we generated a transgenic model in which Smad7 transgene expression can be induced in the epidermis and hair follicles (gene-switch-Smad7) by topically RU486 application. Smad7 induction from day 3 to day 7 after excisional wounding reduced inflammatory responses through suppressing expression of a variety of inflammatory cytokines/chemokines in gene-switch-Smad7 mice as compared to control mice. Meanwhile, overexpression of Smad7 exhibited accelerated re-epithelialization, which correlated with increased expression of metalloproteinases and elevated Erk (extracellular signal-regulated kinases) signaling in the leading epidermal edges in gene-switch-Smad7 wounds compared to control wounds. Smad7 induction from day 7 to day 20 after excisional wounding reduced dermal fibrotic response and angiogenesis in the dermis, resulting in a better tissue repair. We conclude that the effectsof Smad7 on wound healing are likely due to blocking the negative effects of TGFβ on cutaneous wound healing.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1067-1927.2004.0abstractai.x
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  • 3
    Electronic Resource
    Electronic Resource
    Unusual Hf contents in metamorphic zircon from coesite-bearing eclogites of the Dabie Mountains, east-central China: implications for the dating of ultrahigh-pressure metamorphism (2004)
    Oxford, UK : Blackwell Science Inc
    Journal of metamorphic geology 22 (2004), S. 0 
    Details
    ISSN: 1525-1314
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Geosciences
    Notes: Metamorphic zircon from coesite-bearing eclogites in the Dabie Mountains encloses high-P phases, and may have formed at the peak of ultrahigh-pressure (UHP) metamorphism. Morphologically, the metamorphic zircon typically occurs as small, multi-faceted, near-spherical grains with homogeneous internal structure and weak backscattered electron (BSE) luminescence. Geochemically, it is characterized by extremely high and relatively constant contents of hafnium (Hf) and very low contents of Y, U and Th, reflecting the contraction of the zircon lattice under the UHP conditions. High contents of Hf may be characteristic of zircon formed during UHP metamorphism, which has important consequences for interpretation of geochronological results. We propose that the metamorphic zircon extremely enriched in Hf may be used to date the peak of UHP metamorphism that produced the coesite-bearing eclogites in the Dabie Mountains, and potentially in other UHP terranes.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1525-1314.2004.00538.x
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  • 4
    Electronic Resource
    Electronic Resource
    Spectrum and inheritance of resistance to the root-knot nematode Meloidogyne hapla in Rosa multiflora and R. indica (2004)
    Oxford, UK : Blackwell Publishing Ltd
    Plant breeding 123 (2004), S. 0 
    Details
    ISSN: 1439-0523
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: The spectrum and inheritance of resistance to the root-knot nematode Meloidogyne hapla was studied in the diploid species Rosa multiflora and R. indica. The host suitability of seven rose rootstocks, namely five R. multiflora (K2, K1, Floradale, CE63 and CE65) and two R. indica (CE35 and Maroc) clonal accessions, was evaluated using four geographic isolates of M. hapla. Plants grown under greenhouse conditions were tested at high and durable inoculum pressure of nematodes and rated for nematode infestation five months after inoculation on a 0–5 gall index. Different host suitabilities to M. hapla were demonstrated depending on the nematode isolates: in R. multiflora, the clone K2 had a resistant (R) response to all isolates; the clone K1 ranged from intermediate (I) (isolate ‘Canada’) to resistant (other isolates), Floradale was shown to be intermediate, whereas CE63 and CE65 were moderate to good hosts (H). In R. indica, both rootstocks were good to excellent hosts for the isolate Canada but resistant to all three other isolates, thus expressing an isolate-specific resistance. A study on the genetics of resistance in R. multiflora to the M. hapla isolate Canada was then conducted using an incomplete diallel cross involving all previous clones except Floradale. A total of 120 hybrid individuals belonging to several progenies representing the cross combinations R × I, R × H, I × H and H × H were evaluated. Individuals of each progeny generally ranged into a monomodal distribution that suggests polygenic inheritance of resistance. In the family Rosaceae, the differences in the resistance genetics to the meiotic species M. hapla and to the mitotic species, M. arenaria, M. incognita and M. javanica are discussed in relation to the reproductive status of the nematodes.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1046/j.0179-9541.2003.00930.x
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  • 5
    Electronic Resource
    Electronic Resource
    Breeding for high yield and fibre quality in coloured cotton (2004)
    Oxford, UK : Blackwell Publishing Ltd
    Plant breeding 123 (2004), S. 0 
    Details
    ISSN: 1439-0523
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: In the present study, three types of coloured fibre cottons, i.e. white, brown and green, were compared for their fibre quality and yield. The comparison of fibre quality suggested that coloured fibre cotton was inferior as compared with white fibre cotton. To understand the effect of cellulose, mineral elements [nitrogen (N), phosphorus (P) and potassium (K)] and pH of fibre cells on the quality of fibre, these components were studied at different fibre cell developed stages in all three fibre cotton types. The cellulose content is closely associated with the quality of fibre. The higher fibre quality of white fibre cotton might be the result of the high cellulose content in it compared with coloured fibre cotton. A rapid and slow decrease in pH in white and coloured cottons, respectively, might have some effects on fibre elongation. Among the mineral contents, potassium is positively correlated with the fibre quality traits. The pigment development patterns in brown and green fibre cottons are not similar. In green fibre cotton it takes more time to deepen in colour as compared with brown fibre cotton. Possible strategies for the improvement in quality of coloured fibre cotton are discussed. The results of heterosis studies in coloured fibre cotton suggest that heterosis could improve yield and quality of coloured fibre cotton. In the present study, the hybrids between ZJU12A x ZJU05R and ZJU18A x ZJU01R, having an acceptable lint colour types plus better fibre quality and high yield performance, may be exploited further for their heterotic advantages.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1046/j.1439-0523.2003.00938.x
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  • 6
    Electronic Resource
    Electronic Resource
    Mapping quantitative trait loci and expressed sequence tags related to brown planthopper resistance in rice (2004)
    Oxford, UK : Blackwell Publishing Ltd
    Plant breeding 123 (2004), S. 0 
    Details
    ISSN: 1439-0523
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: To map genes responsible for brown planthopper (BPH) resistance in rice, a rice genetic map was constructed based on a recombinant inbred line population from a cross between a BPH-resistant line ‘B5’ and a susceptible variety ‘Minghui 63’. Four quantitative trait loci (QTLs) for BPH resistance were detected. ESTs differentially regulated by BPH feeding were isolated by suppressive subtractive hybridization (SSH) and assigned to chromosomes based on RFLP mapping and searches of the rice genome database. The distribution of ESTs showed some clustering, and some ESTs are related to known QTLs and known BPH resistance genes. These findings suggest that the mapping of differentially induced ESTs may be a useful strategy for the identification of candidate plant defence genes, which could be beneficial in the development of a BPH-resistant rice variety.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1439-0523.2004.01003.x
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  • 7
    Electronic Resource
    Electronic Resource
    Jasmonic acid is involved in the water-stress-induced betaine accumulation in pear leaves (2004)
    Oxford, UK : Blackwell Science Ltd
    Plant, cell & environment 27 (2004), S. 0 
    Details
    ISSN: 1365-3040
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Jasmonic acid (JA) is known to be involved in the response of plants to environmental stresses such as drought, and betaine (glycinebetaine) is an osmopretectant accumulated in plants under environmental stresses including drought. However, it remains currently unclear whether JA is involved in the water-stress-induced betaine accumulation in plant leaves. The present experiment, performed with the whole pear plant (Pyrus bretschneideri Redh. cv. Suli), revealed that the exogenously applied JA induced a significant increase of the betaine level in the pear leaves when the plants were not yet stressed by drought, and when the plants were subjected to water stress, the ‘JA plus drought’ treatment induced a significant higher betaine level than did the drought treatment alone. Meanwhile, the ‘JA plus drought’ treatment induced higher levels of betaine aldehyde dehydrogenase (BADH, E C 1.2.1.8) and activities in the leaves than did the drought treatment alone. These results obtained in the whole plant experiments were supported by the results of detached leaf experiments. In detached leaves JA induced significant increases in betaine levels, BADH activities and BADH protein amounts in a time- and concentration-dependent manner. These data demonstrate that JA is involved in the drought-induced betaine accumulation in pear leaves.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1365-3040.2004.01167.x
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  • 8
    Electronic Resource
    Electronic Resource
    Identification of three F5 gene mutations associated with inherited coagulation factor V deficiency in two Chinese pedigrees (2004)
    Oxford, UK : Blackwell Science Ltd
    Haemophilia 10 (2004), S. 0 
    Details
    ISSN: 1365-2516
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: Summary.  To investigate the molecular defects in two Chinese pedigrees with inherited factor V (FV) deficiency.A 37-year-old male (proband 1) and an 18-month-old boy (proband 2) were diagnosed as inherited coagulation FV deficiency by severely reduced plasma levels of FV activity and antigen. All 25 exons and their flanking sequence of F5 gene were amplified by polymerase chain reaction (PCR) for both probands and the PCR products were directly sequenced. Total RNA was extracted from the peripheral lymphocytes of proband 1 for detecting the changes at mRNA level.The homozygous deletion IVS8 −2A〉G was identified in the F5 gene of proband 1 and complementary DNA (cDNA) analysis revealed the abolishment of the canonical splicing site by the mutation and the activation of the cryptic acceptor site 24 bp upstream instead. The insertion introduced eight additional amino acids (AA) into the FV protein. Two heterozygous mutations of F5 gene were discovered in proband 2. The 2238-9del AG in exon 13 introduced a premature termination code at 689 AA and the substitution of G6410 by T in exon 23 lead to the missense mutation Gly2079Val.Three F5 gene mutations, IVS8 −2A〉G, 2238-9del AG and G6410T, have been identified in two Chinese pedigree with congenital FV deficiency, respectively.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1365-2516.2004.00896.x
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  • 9
    Electronic Resource
    Electronic Resource
    Prothrombin Shanghai: hypoprothrombinaemia caused by substitution of Gla29 by Gly (2004)
    Oxford, UK : Blackwell Science Ltd
    Haemophilia 10 (2004), S. 0 
    Details
    ISSN: 1365-2516
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: Summary.  Prothrombin deficiency is a rare bleeding disorder inherited as an autosomal recessive trait. In this study, we reported a Chinese family with hereditary prothrombin deficiency. The proposita had a prolonged activated partial thromboplastin time (APTT, 71.6 s) and prothrombin time (PT, 28.0 s). The coagulation factors activities were normal except that prothrombin coagulation activity was markedly reduced, and the prothrombin antigen level was moderately decreased. Nucleotide sequencing of amplified DNA revealed a novel mutation, Glu (GAG) to Gly (GGG) at residue 29, which normally undergoes γ-carboxylation within the Gla domain of prothrombin. The proposita was identified as homozygous, while her father, mother and maternal grandmother were heterozygous for the mutation. Gla29 has been demonstrated as one of the key residue for Ca2+-binding, membrane interaction and biological activity of prothrombin.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1046/j.1365-2516.2003.00838.x
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  • 10
    Electronic Resource
    Electronic Resource
    Effect of Processing and Storage on Antioxidant Capacity of Honey (2004)
    Oxford, UK : Blackwell Publishing Ltd
    Journal of food science 69 (2004), S. 0 
    Details
    ISSN: 1750-3841
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition , Process Engineering, Biotechnology, Nutrition Technology
    Notes: : The impact of heat and filtration on the antioxidant capacity of clover and buckwheat honey during storage was analyzed. Processing clover honey did not significantly impact antioxidant capacity (determined by oxygen radical absorbance capacity [ORAC] assay); processing lowered the antioxidant capacity of buckwheat honey (33.4%). The antioxidant capacity of honeys was reduced after 6 mo of storage with no impact of storage temperature or container type detected at the end point of the storage period. Processed and raw clover honey antioxidant capacity decreased about 30%. Processed buckwheat honey decreased 24% in antioxidant capacity, whereas raw buckwheat honey decreased 49%. Antioxidant capacity of processed and raw honeys was similar after storage. Phenolic profiles, peroxide accumulation, 5-(hydroxymethyl)-2-furaldehyde (HMF), gluconic acid, and total phenolics were also analyzed. The impact of storage on antioxidant components of processed and raw honey was complex.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1365-2621.2004.tb15509.x
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