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  • Articles: DFG German National Licenses  (2)
  • Electronic Resource  (2)
  • 1985-1989  (2)
  • Biochemistry and Biotechnology  (1)
  • DNA histogram evaluation  (1)
Source
  • Articles: DFG German National Licenses  (2)
Material
  • Electronic Resource  (2)
Years
Year
  • 1
    Electronic Resource
    Electronic Resource
    Springer
    Journal of cancer research and clinical oncology 114 (1988), S. 559-564 
    ISSN: 1432-1335
    Keywords: Ehrlich ascites tumor ; Flow cytometry ; DNA histogram evaluation ; Tumor growth ; Cell cycle
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary DNA histograms of human tumors should be interpreted together with the type of growth. In order to prove this connection the Ehrlich ascites tumors of 144 mice were investigated by absolute cell counting and flow cytometry. The exponential, transition and steady-state phases of tumor development were defined as types of growth based on the logistic function (Verhulst-Pearl). Flow-cytometric histograms (ethidium-bromide, olivomycin) were evaluated by two methods presupposing lognormal distribution and resulting in the same trend of changes concerning the percentage of cells in the three cell-cycle phases. In exponential growth the histograms show a high S-phase compartment and a decrease of the G2M peak. The increased percentage of S-phase cells is caused by a recruitment from G0-1 and G0-2 after transplantation. The decrease of the G2M peak is due to the exponential growth, a recruitment from G0-2 and a relative shortening of G2M duration. A high G2M peak and a low S-phase portion occur in steady state. Besides a decrease of DNA-synthezising cells the S compartment decreases as a result of a relative S-phase shortening. For the increase of the G2M peak, transition into steady state, prolongation of G2M duration, recruitment of G0-1 and the occurrence of G0-2 cells are responsible. The same histogram may reflect different biological behavior patterns depending on the type of growth.
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 27 (1985), S. 626-631 
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Zymomonas mobilis immobilized on microporous ion exchange resins has previously been shown to allow the attainment of high ethanol productivities in packed-bed bioreactors. The formation of bacterial filaments after several days of continuous operation, however, had resulted in excessive pressure increases across the reactor bed. The present work examines techniques for controlling filament formation by Z. mobilis in two reactor sizes (161 mL and 7.85 L) and a feed glucose concentration of 100 g/L. By controlling the fermentation temperature at 20-25°C it has been possible to eliminate filament formation by Z. mobilis and to operate the larger bioreactor for 232 h with an ethanol productivity of 50 g/L h (based on total reactor volume). The rate of ethanol production has been shown to be very sensitive to temperature in the range 20-30°C, and it is likely that slightly higher temperatures than those used in this study will improve ethanol productivity while still permitting long-term operation.
    Additional Material: 5 Ill.
    Type of Medium: Electronic Resource
    Library Location Call Number Volume/Issue/Year Availability
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