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  • Articles: DFG German National Licenses  (6)
  • Electronic Resource  (6)
  • 1985-1989  (6)
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  • Articles: DFG German National Licenses  (6)
Material
  • Electronic Resource  (6)
Years
Year
Keywords
  • 1
    Electronic Resource
    Electronic Resource
    Oxidative Metabolism of Leukotriene E4 by Rat Hepatocytes (1988)
    Oxford, UK : Blackwell Publishing Ltd
    Annals of the New York Academy of Sciences 524 (1988), S. 0 
    Details
    ISSN: 1749-6632
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Natural Sciences in General
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1749-6632.1988.tb38529.x
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Determination of sulfidopeptide leukotrienes in biological fluids by gas chromatography mass spectrometry (1986)
    s.l. : American Chemical Society
    Analytical chemistry 58 (1986), S. 1098-1101 
    Details
    ISSN: 1520-6882
    Source: ACS Legacy Archives
    Topics: Chemistry and Pharmacology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1021/ac00297a026
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    Paper (German National Licenses)
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  • 3
    Electronic Resource
    Electronic Resource
    Analysis of phospholipid molecular species in rat lung as dinitrobenzoate diglycerides by electron capture negative chemical ionization mass spectrometry (1987)
    Chichester : Wiley-Blackwell
    Biological Mass Spectrometry 14 (1987), S. 573-578 
    Details
    ISSN: 0887-6134
    Keywords: Chemistry ; Analytical Chemistry and Spectroscopy
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: The use of electron capture negative ion desorption chemical ionization mass spectrometry was demonstrated in the analysis of phospholipid molecular species at the 1,3-dinitrobenzoate (DNB) diglyceride derivative. Modification of phosphatidylcholine (PC) or phosphatidylethanolamine (PE) by phospholipase C treatment and acylation of the resultant diglyceride with 3,5-dinitrobenzoylchloride afforded separation of the alkylacyl, alkenylacyl, and diacyl dinitrobenzoate subclasses by thin-layer chromatography (TLC). Separation of alkylacyl DNB into individual molecular species by reverse-phase high-performance liquid chromatography (RPHPLC) was demonstraed. Electron capture desorption chemical ionization of individual molecular species (10-25 ng) from a direct probe yielded a mass spectrum characterized by an intense molecular anion. This molecular anion was the base peak of the spectrum accounting for greater than 80% of the total ionization. From this molecular anion the total carbon number and degree of unsaturation of the fatty chains could be determined. Analysis of fatty acid content of the molecular species allowed unequivocal assignment of structure for the alkyl ether phospholipids. Using selected ion monitoring as little as 0.5 pmol of these species could be detected with a signal-to-noise ratio ≥3. This technique was useful in the analysis of low picomolar amounts of molecular species of ether phospholipids in the rat lung. Given an appropriate internal standard, analysis of dynamic changes in turnover, metabolism and precursor product relationships could be undertaken.
    Additional Material: 5 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/bms.1200141007
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    Paper (German National Licenses)
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  • 4
    Electronic Resource
    Electronic Resource
    Electron capture negative ion chemical ionization analysis of arachidonic acid (1988)
    Chichester : Wiley-Blackwell
    Biological Mass Spectrometry 15 (1988), S. 175-178 
    Details
    ISSN: 0887-6134
    Keywords: Chemistry ; Analytical Chemistry and Spectroscopy
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: A highly sensitive (subpicomole level) and structural specific method for the analysis of arachidonic acid esterified to complex glycerophospholipids has been developed using combined capillary gas chromatography and mass spectrometry. The methodology is based upon the formation of the pentafluorobenzyl ester of arachidonic acid, which is efficiently ionized using electron capture negative ion chemical ionization conditions to yield an abundant carboxylate anion at m/z 301. Quantification is carried out following hydrolysis of the complex glycerophospholipid in the presence of a known amount of (2H8) araachidonic acid. The use of this method is illustrated by the quantification of arachidonic acid within the glycerophospholipid classes isolated from resident peritoneal macrophage cells isolated from HS mice.
    Additional Material: 3 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/bms.1200150309
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  • 5
    Electronic Resource
    Electronic Resource
    Fast atom bombardment analysis of arachidonic acid-containing phosphatidylcholine molecular species (1986)
    Chichester : Wiley-Blackwell
    Biological Mass Spectrometry 13 (1986), S. 71-76 
    Details
    ISSN: 1052-9306
    Keywords: Chemistry ; Analytical Chemistry and Spectroscopy
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: Fast atom bombardment (FAB) mass spectrometry was evaluated as a means for the characterization of molecular species of glycerophosphocholines (GPC) from HL60 cells. Previous reports of phospholipid molecular species analysis have suggested that several inherent problems with FAB could limit its analytical usefulness in such an application. The GPC-related secondary ions produced by the FAB experiment were found to be dependent on the matrix employed. Triethanolamine was found to minimize mass-related discrimination in ion emission when mixtures were studied; and furthermore, this matrix maintained 60% maximal ion current after 12 minutes as compared to a glycerol matrix which diminished to 10% maximal ion current. Using triethanolamine, the major GPC species in HL60 cells prelabeled with (2H8) arachidonic acid were found to be 16:0a16:1, 16:0e/18:1, 16:0a/18:1, 18:1a/18:1 and 18:0a/18:1.† It was possible to identify the minor GPC species containing arachidonic acid only after partial purification by reverse-phase high-performance liquid chromatography. Comparison of the 2H8/2H0 enrichment data estimated by FAB with data obtained by gas chromatographic/mass spectral analysis of arachidonic acid following GPC hydrolysis revealed that the precision of FAB was less precise than gas chromatography/mass spectrometry. Yet the FAB technique did allow the observation of one unexpected molecular species (18:1a/20:4) due to the fact that the GPC was not degraded to simpler species prior to analysis. In this respect, the two strategies of molecular species analysis complement each other.
    Additional Material: 4 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/bms.1200130205
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    Paper (German National Licenses)
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  • 6
    Electronic Resource
    Electronic Resource
    Preparation of oxygen-18-labeled lipoxygenase metabolites of arachidonic acid (1985)
    Chichester : Wiley-Blackwell
    Biological Mass Spectrometry 12 (1985), S. 714-718 
    Details
    ISSN: 1052-9306
    Keywords: Chemistry ; Analytical Chemistry and Spectroscopy
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: Plasma pseudocholinesterase and porcine liver esterase were used to catalyse the incorporation of the stable isotope oxygen-18 into the carboxyl moiety of lipoxygenase metabolites of arachidonic acid. This simple method produces eicosanoid products containing two oxygen-18 atoms; but the enzymes studied were found to display large substrate specificity in the efficiencies at which oxygen-18 could be incorporated into the lipoxygenase metabolites. Furthermore, [18O2]LTB4 was found not to back exchange during in vitro incubation with human neutrophils. The methods involved for stable isotope incorporation are simple, efficient and produce highly enriched species in a short time. By varying the type of esterase, the amount of esterase or the length of incubation highly enriched species of all eicosanoids tested could be prepared.
    Additional Material: 3 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/bms.1200121208
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    Paper (German National Licenses)
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