ISSN:
1750-3841
Source:
Blackwell Publishing Journal Backfiles 1879-2005
Topics:
Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
,
Process Engineering, Biotechnology, Nutrition Technology
Notes:
Electrophoretic methods for detecting small proportions of contaminating species in fresh or frozen beef samples were investigated. By staining isoelectric focusing gels for particular enzymes, rather than for proteins in general, lower proportions of contaminants could be detected, because the band patterns were less complex. Buffalo, horse and pig muscles possess phosphoglucomutase isozymes of higher isoelectric point that beef, and this enabled detection of these species at contaminant:beef ratios as low as 1:99. By staining for adenylate kinase, kangaroo and horse were detected in beef samples at similar concentrations. Enzyme stains also proved useful for distinguishing some species not differentiated by general-protein stains, e.g. phosphogluconate dehydrogenase distinguishes sheep from goat.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1111/j.1365-2621.1982.tb04993.x
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