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Temperature-sensitive nuclear mutants of Neurospora crassa deficient in mitochondrial ribosomes (1979)

Nargang, Frank E. ; Bertrand, Helmut ; Collins, Richard A.

Springer

Current genetics 1 (1979), S. 1-7

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ISSN: 1432-0983

Keywords: Mitochondrial rRNA ; Nuclear/mitochondrial interactions

Source: Springer Online Journal Archives 1860-2000

Topics: Biology

Notes: Summary We have isolated two non-allelic nuclear mutants of Neurospora crassa that are temperature-sensitive for the production of cytochromes aa 3 and b. When grown at 23 °C the mutants are virtually indistinguishable from the parent wild-type strains. When grown at 41 °C the mutants have large amounts of KCN-insensitive respiration and lack cytochromes aa 3 and b. Further examination of the mutants revealed that they were extremely deficient in their capacity for mitochondrial protein synthesis when grown at 41 °C. This protein synthesis deficiency appears to be related to a virtual absence of both small and large mitochondrial ribosomal subunits following growth at 41 °C. Examination of the mitochondrial RNAs of the mutants suggests that mitochondrial rRNAs are synthesized in greatly reduced amounts or that they are misprocessed when these mutants are grown at the non-permissive temperature.

Type of Medium: Electronic Resource

URL: http://dx.doi.org/10.1007/BF00413301

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Hartree-like methods in electronic structure theory (1978)

Harris, Frank E.

New York, NY : Wiley-Blackwell

International Journal of Quantum Chemistry 13 (1978), S. 189-198

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ISSN: 0020-7608

Keywords: Computational Chemistry and Molecular Modeling ; Atomic, Molecular and Optical Physics

Source: Wiley InterScience Backfile Collection 1832-2000

Topics: Chemistry and Pharmacology

Notes: We consider the “Hartree Problem,” here defined as the problem of finding the orthogonal one-electron states which in a single determinant minimize the electronic energy, excluding exchange contributions. These states may provide a useful basis for correlation-energy studies. It is shown how the “Hartree Problem,” although superficially not of pseudoeigenvalue form, can nevertheless be cast in such a form and solved by an iterative diagonalization process.