ISSN:
1432-2013
Keywords:
Intestinal Absorption
;
Medium Chain Fatty Acids
;
Kinetics
;
Active Transport
;
Rat
Source:
Springer Online Journal Archives 1860-2000
Topics:
Medicine
Notes:
Summary 1. The transport of14C-octanoate by rat small intestine was determined in vitro by a modification of the method of Semenza and Mühlhaupt, and in vivo by using isolated segments or a perfusion technique in conscious rats (Haberichet al., 1968). 2. The absorption capacity of14C-octanoate in the distal ileum was significantly lower than in the proximal jejunum. 3. 14C-octanoate injected into a jejunal segment appeared rapidly in portal venous blood reaching its maximal concentration 4 min after the injection. 4. The transport of octanoate showed a saturation effect with increasing concentrations after an incubation of 4 min. However, with constant concentrations of octanoate in the medium, the transport rate was constant for 8–10 min. A similar saturation effect was also demonstrated in vivo. Values for maximal absorption rate (V max) and the half-saturation constant (K m), calculated from the Lineweaver-Burk plot, were 1.0 μMol/min and 17.7 mM, respectively. 5. Accumulation of octanoate in tissue water was demonstrated. For example, after incubation for 40 min in a medium concentration of 0.1 mM, the tissue/ medium ratio was 2.75:1. 6. Cyanide (2×10−4 M) markedly diminished the absorption rate of octanoate. 7. The absorption of octanoate was competitively inhibited by caproic acid (C6) in vitro. The inhibition constant (K i), calculated from the Dixon plot, was 47 mM. 8. Simultaneous administration of glucose and octanoate in vivo did not affect the absorption rate of the fatty acid. 9. These results suggest that the intestinal absorption of octanoate is an energy-dependant active process.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00592157
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