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  • Articles: DFG German National Licenses  (25)
  • 1990-1994  (25)
  • 1993  (13)
  • 1991  (12)
  • 1
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    Plant pathology 42 (1993), S. 0 
    ISSN: 1365-3059
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: The infection of pea (Pisum sativum) by Colletotrichum truncatum was studied by light and electron microscopy. These investigations were facilitated by use of an Argenteum pea mutant, which has a readily detachable epidermis. Infection pegs emerging from appressoria penetrated epidermal cells directly. Large intracellular primary hyphae formed a dense stromatic mycelium confined within a single epidermal cell. Primary mycelia gave rise to thinner secondary hyphae which radiated into surrounding cells and caused extensive wall dissolution. Melanized sclerotia developed in the centre of chlorotic water-soaked lesions. Acervuli were not observed. Epidermal cells survived initial penetration by primary hyphae, as shown by their ability to plasmolyse and accumulate Neutral red, but all infected cells were dead when the secondary hyphae had formed. Six cultivars of pea were susceptible, but seven other legumes were resistant. A single isoform of polygalacturonase with a pI of 8·3 and apparent Mr of 40000 was purified from culture filtrates and the TV-terminal amino acid sequence determined. The relevance of the results to the taxonomy of C. truncatum and the relationships between infection process and host range are discussed.
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    Weed research 31 (1991), S. 0 
    ISSN: 1365-3180
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: An in-vitro growth system for investigating the behaviour of S. gesnerioides on cowpea has been developed. The roots of young cowpea plants were spread over glass fibre filter paper in a shallow plastic tray, and a known number of germinated Striga seeds were placed on the cowpea root surface. This allowed the infection process of the parasite to be quantified. Good access to the host-parasite association enabled the entire infection process to be easily monitored and tissue removed for cytological studies. Observations revealed for the first time that contact of S. gesnerioides radicles with host roots stimulated the development of radicular hairs and swelling of the tip of these radicles. Furthermore, penetration of cowpea roots by Striga was shown to be localized and to cause very little damage to the host roots. Swollen parasite tubercles developed on the surface of the roots only after the host stele had been penetrated by invading Striga cells. This growth system is suitable for in-vestigating the mechanisms of host resistance. and also for rapidly screening cowpea germplasm for new sources of resistance to the Parasite. Resistant cowpea cultivars offer the best potential solution for control of this Parasite.
    Type of Medium: Electronic Resource
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  • 3
    Electronic Resource
    Electronic Resource
    Oxford [u.a.] : International Union of Crystallography (IUCr)
    Acta crystallographica 49 (1993), S. 684-687 
    ISSN: 1600-5759
    Source: Crystallography Journals Online : IUCR Backfile Archive 1948-2001
    Topics: Chemistry and Pharmacology , Geosciences , Physics
    Type of Medium: Electronic Resource
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  • 4
    Electronic Resource
    Electronic Resource
    Oxford [u.a.] : International Union of Crystallography (IUCr)
    Acta crystallographica 49 (1993), S. 793-796 
    ISSN: 1600-5759
    Source: Crystallography Journals Online : IUCR Backfile Archive 1948-2001
    Topics: Chemistry and Pharmacology , Geosciences , Physics
    Type of Medium: Electronic Resource
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  • 5
    Electronic Resource
    Electronic Resource
    Oxford [u.a.] : International Union of Crystallography (IUCr)
    Acta crystallographica 49 (1993), S. 1598-1602 
    ISSN: 1600-5759
    Source: Crystallography Journals Online : IUCR Backfile Archive 1948-2001
    Topics: Chemistry and Pharmacology , Geosciences , Physics
    Type of Medium: Electronic Resource
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  • 6
    Electronic Resource
    Electronic Resource
    s.l. ; Stafa-Zurich, Switzerland
    Solid state phenomena Vol. 32-33 (Dec. 1993), p. 141-142 
    ISSN: 1662-9779
    Source: Scientific.Net: Materials Science & Technology / Trans Tech Publications Archiv 1984-2008
    Topics: Physics
    Type of Medium: Electronic Resource
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  • 7
    Electronic Resource
    Electronic Resource
    Springer
    Experiments in fluids 15 (1993), S. 10-16 
    ISSN: 1432-1114
    Source: Springer Online Journal Archives 1860-2000
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
    Notes: Abstract This paper describes and evaluates a thermal transient anemometer, a novel fluid flow (mass flux) measuring device having a thermocouple probe which is utilized to measure the change in temperature over a period of time to provide a measure of fluid flow velocity. The thermocouple probe (sensor) is periodically heated by the application of an electrical pulse and its rate of cooling between pulses is related to the local mean flow velocity. The theory of operation, device description, comparison with other similar fluid flow devices, and laboratory tests including measurements under different turbulence levels and different fluid temperatures are presented and discussed.
    Type of Medium: Electronic Resource
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  • 8
    Electronic Resource
    Electronic Resource
    Springer
    Bioprocess and biosystems engineering 6 (1991), S. 273-284 
    ISSN: 1432-0797
    Source: Springer Online Journal Archives 1860-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Notes: Abstract Manipulation of cellular metabolism to maximize the yield and rate of formation of desired products may be achieved through genetic modification. Batch fermentations utilizing glucose as a carbon source were performed for three recombinant strains of Saccharomyces cerevisiae in which the glucose phosphorylation step was altered by mutation and genetic engineering. The host strain (hxk1 hxk2 glk) is unable to grow on glucose or fructose; the three plasmids investigated expressed hexokinase PI, hexokinase PII, or glucokinase, respectively, enabling more rapid glucose and fructose phosphorylation in vivo than that provided by wild-type yeast. Intracellular metabolic state variables were determined by 31P NMR measurements of in vivo fermentations under nongrowth conditions for high cell density suspensions. Glucose consumption, ethanol and glycerol production, and polysaccharide formation were determined by 13C NMR measurements under the same experimental conditions as used in the 31P NMR measurements. The trends observed in ethanol yields for the strains under growth conditions were mimicked in the nongrowth NMR conditions. Only the strain with hexokinase PI had higher rates of glucose consumption and ethanol production in comparison to healthy diploid strains in the literature. The hexokinase PII strain drastically underutilized its glucose-phosphorylating capacity. A regulation difference in the use of magnesium-free ATP for this strain could be a possible explanation. Differences in ATP levels and cytoplasmic pH values among the strains were observed that could not have been foreseen. However, cytoplasmic pH values do not account for the differences observed among in vivo and in vitro glucose phosphorylation activities of the three recombinant strains.
    Type of Medium: Electronic Resource
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  • 9
    Electronic Resource
    Electronic Resource
    Springer
    Plant and soil 135 (1991), S. 185-196 
    ISSN: 1573-5036
    Keywords: calcium ; critical phosphorus concentration ; gypsum ; lime ; perennial ryegrass ; root membranes ; soluble carbohydrate ; zinc
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract The results of a previous study had suggested that under conditions of limited P availability, Ca may be able to compensate for P in the shoot tissue of perennial ryegrass. To verify this preliminary finding, a factorial experiment was set up which simultaneously tested the effects of Ca and P fertilization on the yield and chemical composition of perennial ryegrass. Calcium was supplied as either lime or gypsum in order to differentiate between the effects of Ca and pH on the response of perennial ryegrass to P fertilization. In the final stage of the experiment a Zn treatment was included, to see whether altering the P/Zn ratios of plant shoots had any influence on the purported interaction between Ca and P. The results demonstrated that the P-sparing effect of lime occurs, at least partly, because Ca application improves the efficiency of absorbed P for DM production. However, it was reasonably clear that the site of the interaction between Ca and P was the soil-root interface, and not shoot tissue. It was suggested that under conditions of limited P supply, Ca stablizes root membranes and thereby minimizes both the efflux losses of nutrients from root tissue, and the compensatory flow of photosynthates from shoots to roots. No interaction was observed between P and Zn treatments in this study. Instead, a positive interaction was found between lime and Zn treatments, which suggests that the stabilizing action of Ca on root membranes requires Zn as a co-stabilizing factor. It is proposed that chemical analysis of shoot tissue alone may not be sufficient to accurately diagnose the P, Ca or Zn status of whole plants, since the critical levels of these elements in shoots appear to bear little relation to their requirements in the rhizosphere.
    Type of Medium: Electronic Resource
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  • 10
    ISSN: 1573-5060
    Keywords: Striga gesnerioides ; Vigna unguiculata ; cowpea ; parasitic angiosperm ; breeding for resistance ; parasite variation ; races
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract An in vitro growth system was used to determine the virulence of two samples of Striga gesnerioides from Zakpota in southern Benin. Cowpea variety B301, previously considered resistant to all races of S. gesnerioides, was susceptible to both samples of the parasite. Two other cowpea varieties, 58–57 and IT81D-994, were totally resistant. Resistance in 58–57 was associated with a hypersensitive necrosis of infected roots, whilst IT81D-994 supported production of small S. gesnerioides tubercles with stems which failed to develop. Striga gesnerioides from southern Benin is the fourth race of the parasite to be identified, and the first with virulence on variety B301. The implications for breeding cowpeas with resistance to S. gesnerioides are discussed.
    Type of Medium: Electronic Resource
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