ISSN:
1573-5079
Keywords:
photosynthetic electron transport
;
structure and function relationship
;
lipids
;
α-tocopherol
;
free-flow electrophoresis
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract A chloroplast preparation was extracted from squash (Cucurbita pepo (L.) var. Senator). Enrichment of intact chloroplasts was achieved by continuous free-flow electrophoresis. The addition of monoterpenes, detergent and free fatty acids changed the elecrophoretic separation pattern characteristically. Monoterpene-dependent degradation of envelope membranes could be prevented by addition of α-tocopherol prior to monoterpene incubation. Photosynthetic electron transport of photosystem II was completely inhibited by β-pinene, Triton X-100 and linolenic acid. Inhibition could be modulated by addition of α-tocopherol or lecithin (phosphatidylcholine) either before or after inhibition by monoterpenes and detergent. Percentage reconstitution of photosynthetic electron transport inhibited by β-pinene depended on light conditions and incubation time.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00054124
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