ISSN:
1573-4994
Keywords:
lin-Benzo-ATP
;
lin-benzo-ADP
;
dissociation constants
;
Mg2+ and Ca2+ binding
;
fluorescence spectra
Source:
Springer Online Journal Archives 1860-2000
Topics:
Physics
Notes:
Abstract lin-Benzo-adenine nucleotides can act not only as probes for fluorescence studies but also as structural active site probes for enzymes. To understand the basic properties oflin-benzo-ATP and-ADP, protolysis and Mg2+ and Ca2+, binding are investigated between pH 6.2 and pH 8.5 by spectrophotometric and spectrofluorometric titrations. Based on a reaction model, a set of equilibrium constants is determined which is consistent with all available experimental results. The pK values of the Mg2+ and Ca2+ complex oflin-benzo-ATP in the chosen medium are 4.6 and 4.1, respectively, and those for the corresponding diphosphate are 3.1 and 2.8, respectively. Fluorescence and absorption spectra are reported.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF01878458
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