ISSN:
1612-1112
Keywords:
Capillary zone electrophoresis
;
Chiral separation
;
Chiral selectors
;
Dextran and dextrin
;
Drugs
Source:
Springer Online Journal Archives 1860-2000
Topics:
Chemistry and Pharmacology
Notes:
Summary Polysaccharides such as dextrins, which are mixtures of linear α-(1,4)-linked D-glucose polymers, and dextrans, which are polymers of D-glucose units linked predominantly by α-(1,6) bonds, have been employed as chiral selectors in the capillary electrophoretic (CE) separation of enantiomers. Because these polymers are electrically neutral, the method is applicable to ionic compounds. Among the compounds tested the enantiomers of ibuprofen, naproxen, warfarin and the diltiazem synthetic intermediate 1,1′-binaphthyl-2,2′diyl hydrogen phosphate, which are anionic compounds, were successfully recognized by employing dextran or dextrin under neutral conditions. The enantiomers of basic or cationic drugs such as timepidium, primaquine, sulconazole, trimetoquinol, diltiazem and clentiazem were also successfully separated under acidic conditions. The effects of molecular mass and polysaccharide concentration on enantioselectivity were investigated. High concentrations of these polymers, especially dextrin, led to delayed migration times after repeated injections of the solutes into an uncoated capillary under neutral conditions. Consequently the capillary washing process was found to be very important to obtaining reproducible migration times in the system. A coated capillary was also investigated to overcome this problem. Finally, the method using electrically neutral polysaccharides was applied to the testing of the optical purity of the drug substances.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF02267693
Permalink
