ISSN:
1432-2013
Keywords:
Epithelial transport
;
Basolateral membrane
;
K+ channels
;
Quinidine
;
Tadpole skin
;
Noise analysis
Source:
Springer Online Journal Archives 1860-2000
Topics:
Medicine
Notes:
Abstract The skin of frog larvae (Rana catesbeiana) was used to study the characteristics of basolateral K+ channels with fluctuation analysis. K2SO4 and Na2SO4 Ringer's were used as mucosal and serosal solution, respectively. After addition of Nystatin (138 U/ml) the transepithelial conductance and short-circuit current (I sc) increased considerably. Most ofI sc was carried by K+, moving from the mucosal to the serosal side. This current could be depressed by quinidine, added to both compartments or to mucosal side only. Fluctuation analysis showed that quinidine induced a Lorentzian component in the power density spectrum. Assuming pseudo-first order kinetics for the channel occlusion by quinidine the current through the open K+ channel and channel density were calculated:i K=0.22 pA, M=7.7 channels/μm2.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00581784
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