ISSN:
1432-203X
Keywords:
Anther culture
;
Brassica alba
;
primary and secondary embryoids
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract In vitro culture ofBrassica alba anthers on a growth medium containing inorganics of KB5 and organics, iron, sucrose and hormones of B5 resulted in a very high response of anthers (93.75%) towards callus induction. All the calli transferred to regeneration media responded favourably even after six months of callus induction. Numerous torpedo-shaped embryoids developed in clusters at many sites from each callus mass. Secondary embryogenesis and multiple shoot formation was also observed in many cases. The number of embryoids and plantlets produced by one embryogenic anther were as high as 169.8 and 17 respectively. 87% of the regenerated plants were haploids.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF02441010
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