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  • Articles: DFG German National Licenses  (7)
  • 1995-1999  (5)
  • 1990-1994  (2)
  • 1
    Electronic Resource
    Electronic Resource
    Light effects on in vitro adventitious root formation in axillary shoots of mature Prunus serotina (1990)
    Oxford, UK : Blackwell Publishing Ltd
    Physiologia plantarum 80 (1990), S. 0 
    Details
    ISSN: 1399-3054
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Light effects on in vitro adventitious root formation in axillary shoots of a 95-year-old black cherry (Prunus serotina Ehrh.) were examined using microcuttings derived from cultured vegetative buds. Three studies were performed: 1) complete darkness and 4 levels of continuous white light irradiance were tested at 70, 278, 555 and 833 μmol m−2 s−1; 2) white, red, yellow and blue light were tested to assess the importance of spectral quality; and 3) the effect of blue light at intensities of 7,15, 22 and 30 μmol m−2 s−1 was also studied, Measurements included rooting percentage, total number of roots per shoot, and shoot and root dry weight. There was a strong negative effect of white light intensity upon root formation. Blue light between 15 and 22 μmol m−2: s−1 significantly retarded root formation and completely inhibited it at 36 μmol m−2 s−1. Shoots treated with yellow light exhibited the highest rooting percentage, mean number of roots per shoot, and root dry weight.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1399-3054.1990.tb00050.x
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Computergestützte Auswertung eines In-vitro-Transformationstests (1999)
    Springer
    Bundesgesundheitsblatt, Gesundheitsforschung, Gesundheitsschutz 42 (1999), S. 717-721 
    Details
    ISSN: 1437-1588
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Description / Table of Contents: Summary The methodology of transformation assays in in vitro carcinogenesis has been continuously improved to make them routinely applicable. In most of the recently employed transformation assays colony counting was performed under a stereo microscope. We have investigated the application of a computerized image analysing system to determine transformed colonies after in vitro cultivation of epithelial canine kidney cells. Special intention was imposed on the calibration of the system. First results show that determination of number and surface of transformed colonies in soft agar were rapidly available. In addition a high level of reproducibility and a thereby enhanced reliability could be achieved. The implementation of such a computerized image analysis in routine performance of transformation assays would enhance its robustness and applicability and minimize the bias due to evaluation under the stereo microscope to an important extent. It may also be used for similar purpos (e.g. cytogenetic tests).
    Notes: Zusammenfassung Transformationstests als In-vitro-Nachweis von Kanzerogenen werden seit einiger Zeit zur Anwendungsreife entwickelt. Bei der Auswertung solcher Tests werden transformierte Zellkolonien mit der Stereolupe ausgezählt. Wir haben in einem Transformationstest, mit epithelialen Nierenzellen vom Hund als Zielzellen, die Möglichkeit einer automatisierten Auswertung von transformierten Kolonien im Soft Agar untersucht. Dazu wurde die Anzahl und Größe der im Soft Agar gewachsenen Kolonien durch Einsatz eines computergestützten Bildverarbeitungsprogrammes bestimmt. Die Ergebnisse nach der Kalibrierung des Systems zeigen, daß mit der hier verwendeten Methodik schnell und reproduzierbar die Anzahl der Kolonien und auch die Koloniefläche bestimmt werden können. Dadurch ist ein hohes Maß an Genauigkeit und eine Minimierung des subjektiven Auswertungsfehlers erreichbar. Diese computergestütze Auswertmethodik, die prinzipiell auch für andere Anwendungszwecke in Frage kommt, macht einen routinemäßigen Einsatz von Transformationstests erheblich kostengünstiger.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s001030050191
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    Paper (German National Licenses)
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  • 3
    Electronic Resource
    Electronic Resource
    DNA-mediated transformation of a fungus employing a vector devoid of bacterial DNA sequences (1995)
    Springer
    Applied microbiology and biotechnology 43 (1995), S. 1077-1081 
    Details
    ISSN: 1432-0614
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Abstract Acremonium chrysogenum, a producer of cephalosporin C, was subjected to DNA-mediated transformaations using a vector without bacterial DNA sequences. Recombinant fungal strains were generated with a gel-purified DNA fragment, carrying only the mutated β-tubulin gene from A. chrysogenum. The lack of any bacterial DNA was verified by Southern hybridization analysis and polymerase chain reaction amplifications to detect even residual DNA sequences. This procedure can be referred to as a self-cloning experiment for which less restricted working regulations are needed. Finally, the transfer of a synthetic hirudin gene by contransformation demonstrated that any DNA molecule can be introduced into the A. chrysogenum genome without bacterial marker genes. This seems to be highly relevant for biotechnical processes in which safe recombinant producer strains are required to satisfy governmental restrictions.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00166928
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    Paper (German National Licenses)
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  • 4
    Electronic Resource
    Electronic Resource
    Electronic Band Structure of Monolayer Bi on GaP(110) (1995)
    Chester : International Union of Crystallography (IUCr)
    Journal of synchrotron radiation 2 (1995), S. 256-260 
    Details
    ISSN: 1600-5775
    Source: Crystallography Journals Online : IUCR Backfile Archive 1948-2001
    Topics: Geosciences , Physics
    Notes: The two-dimensional electronic band structure of monolayer Bi on GaP(110) has been mapped using angle-resolved UV photoelectron spectroscopy (ARUPS) with synchrotron radiation. Surface photovoltage effects are corrected for by simultaneous second-order core spectroscopy. From valence-band spectra along the four symmetry directions of the surface Brillouin zone at three photon energies it is possible to distinguish three states as surface related. The topmost band is found to be inside the fundamental band gap, at ca 0.75 eV above the bulk valence-band maximum. Comparison with other V/III-V(110) systems shows that this system is not significantly different, despite the relatively large size of the Bi atoms with respect to the GaP lattice; in a selective comparison with InP and GaAs it would appear that Bi-substrate anion bonding is a more important factor than strain.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1107/S0909049595009770
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    Paper (German National Licenses)
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  • 5
    Electronic Resource
    Electronic Resource
    Observation of small metastable multiply charged CsI clusters embedded inside rare gas clusters (1999)
    Springer
    The European physical journal 9 (1999), S. 273-276 
    Details
    ISSN: 1434-6079
    Keywords: PACS: 36.40.Qv Stability and fragmentation of clusters – 36.40.Wa Charged clusters
    Source: Springer Online Journal Archives 1860-2000
    Topics: Physics
    Notes: Abstract. Medium size Kr and Ar clusters 〈N〉∼200-400 were used to pick up and aggregate CsI molecules. By varying the number of pick-up events it was possible to change the size of the guest cluster and the thickness of the rare gas coverage around the alkali halide cluster. “Soft” single (multiple) ionisation of the alkali halide core was achieved via charge transfer from the primary ionised rare gas shell. It turns out that the rare gas shell controls the vibrational relaxation of CsI cluster ions. Due to cooling by the rare gas shell shallow metastable states of multiply charged CsI clusters can be populated and detected. Clusters as small as Cs5I3 2+, Cs6I5 2+, Cs13I13 3+ and Cs17I14 3+ could be observed for the first time. The abundance of these multiply charged clusters steeply decreases if the thickness of rare gas shell shrinks.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s100530050439
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    Paper (German National Licenses)
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  • 6
    Electronic Resource
    Electronic Resource
    Aggregation of small CsI clusters inside Ar clusters: ionization and fragmentation under soft X-ray excitation (1999)
    Springer
    The European physical journal 9 (1999), S. 277-281 
    Details
    ISSN: 1434-6079
    Keywords: PACS: 36.40.Qv Stability and fragmentation of clusters – 36.40.Mr Spetroscopy and geometrical structure of clusters – 78.70.Dm X-ray absorbtion spectra
    Source: Springer Online Journal Archives 1860-2000
    Topics: Physics
    Notes: Abstract. Medium size Ar clusters 〈N〉∼500 were used to pick-up and aggregate CsI molecules. The primary Ar cluster beam can be converted into a beam of bare or Ar-covered alkali halide clusters by varying the number of pick-up events. Soft X-ray photoionisation was used to monitor the depletion of the Ar shell around the guest cluster. It was found that the Ar coverage controls the mechanism and degree of guest cluster ionization. For embedded CsI clusters charge transfer from the Ar shell is the dominant soft X-ray ionization process. Small alkali halide cluster fragments are ejected from the Ar host cluster. The detection of weakly bound stoichiometric (CsI)n + clusters indicates that the Ar-shell prevents the desorption of neutral halogen atoms.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s100530050440
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    Paper (German National Licenses)
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  • 7
    Electronic Resource
    Electronic Resource
    038 Identification of 20 kDa-selenoproteins in the testis and other tissues of the rat (1992)
    Springer
    Fresenius' Zeitschrift für analytische Chemie 343 (1992), S. 92-92 
    Details
    ISSN: 1618-2650
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00332032
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    Paper (German National Licenses)
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