ISSN:
0006-3592
Keywords:
ultrafiltration
;
proteins
;
colloidal interactions
;
Chemistry
;
Biochemistry and Biotechnology
Source:
Wiley InterScience Backfile Collection 1832-2000
Topics:
Biology
,
Process Engineering, Biotechnology, Nutrition Technology
Notes:
A rigorous dynamic mathematical model for predicting the rate of ultrafiltration of proteins has been developed. The model is based on sophisticated descriptions of the protein-protein interactions within the layer close to the membrane surface which are responsible for controlling permeation rate. Electrostatic interactions are accounted for by a Wigner-Seitz cell approach, including a numerical solution of the nonlinear Poisson-Boltzmann equation. London-van der Waals forces are calculated using a computationally efficient means of approximating screened, retarded Lifshitz-Hamaker constants. Configurational entropy effects are calculated using an equation of state giving excellent agreement with molecular dynamic data. Electroviscous effects are also taken into account. These descriptions of protein-protein interactions are used to develop an a priori model, with no adjustable parameters, that allows quantitative prediction of the rate of filtration of proteins as a function of zeta potential (and hence pH), ionic strength, applied pressure, protein size, and membrane resistance. A comparison with experimental data for the filtration of bovine serum albumin (BSA) shows that the model is in excellent agreement with such data. © 1996 John Wiley & Sons, Inc.
Additional Material:
8 Ill.
Type of Medium:
Electronic Resource
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