ISSN:
1432-2013
Keywords:
Key words Gastric smooth muscle
;
Calcium current
;
Actin microfilament
;
Osmotic stretch
Source:
Springer Online Journal Archives 1860-2000
Topics:
Medicine
Notes:
Abstract Using the whole-cell patch clamp technique, the role of actin microfilament in hyposmotic increase of voltage-operated calcium channel current (I Ba) was studied in guinea-pig gastric myocytes. Hyposmotic superfusate (212 mOsm) increased peak I Ba amplitude by 32.7 ± 6.5%; when cytochalasin-D (Cyt-D, 20 μM), an actin cytoskeleton disruptor, was used, an increase of only 9.7 ± 3.1% was seen. I Baresponse to osmotic stress was potentiated (45.1 ± 4.1% increase) by 20 μM phalloidin, an actin microfilament stabilizer. However, colchicine (100 μM), an microtubule cytoskeleton disruptor, had no effect on either I Ba or its response to hyposmotic solution. Phalloidin also induced a rightward shift of the I/V relationship of I Ba, while Cyt-D itself had no effect. These results suggest that actin cytoskeleton may mediate hyposmotic stretch-induced I Ba increase in gastric smooth muscle.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/s004240050428
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