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  • Articles: DFG German National Licenses  (5)
  • 1995-1999  (5)
  • Nonselective cationic current  (3)
  • Enterobacter agglomerans  (2)
  • 1
    Electronic Resource
    Electronic Resource
    Effects of myosin light chain kinase inhibitors on carbachol-activated nonselective cationic current in guinea-pig gastric myocytes (1997)
    Springer
    Pflügers Archiv 434 (1997), S. 346-353 
    Details
    ISSN: 1432-2013
    Keywords: Key words Smooth muscle ; Nonselective cationic current ; Carbachol ; Myosin light chain kinase ; ML-7
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract  The effects of myosin light chain kinase inhibitors on muscarinic stimulation-activated nonselective cationic current (I CCh) in guinea-pig gastric antral myocytes were studied using the whole-cell patch-clamp technique. I CCh was induced by carbachol (CCh, 50 μM) at a holding potential of –30 mV or –60 mV. ML-7, a chemical inhibitor of myosin light chain kinase (MLCK), inhibited I CCh concentration dependently in a reversible manner (53 ± 8.6% at 1 μM, mean ± SE, n = 11). In addition, amplitudes of I CCh were only 37 ± 2.7% of the daily control values following the addition of a peptide inhibitor of MLCK to the pipette solution. On the other hand, ML-7 had an inhibitory effect on voltage-operated Ca2+ channel current. The peak value of Ba2+ current at 0 mV was reduced to 35 ± 7.4% (n = 9) by 3 μM of ML-7. As I CCh is known to have an intracellular Ca2+ dependence, we tried to exclude the possibility that ML-7 inhibited I CCh indirectly via suppression of Ca2+ current and the similar inhibitory effects of ML-7 on I CChwere confirmed under the following conditions: (1) clamp of membrane potential at –60 mV; (2) clamp of intracellular [Ca2+] to 1 μM by 10 mM BAPTA; (3) pre-inhibition of Ca2+ channel by verapamil. Different from the effects on I CCh, ML-7 barely inhibited the same cationic current induced by guanosine 5’-O-(3-thiotriphosphate) (GTP[γS], 0.2 mM) in the pipette solution. These results suggest that a Ca2+/calmodulin-MLCK-dependent pathway can modulate the activation of I CCh in guinea-pig gastric antral myocytes.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s004240050407
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Inhibitory effect of phorbol 12, 13 dibutyrate on carbachol-activated nonselective cationic current in guinea-pig gastric myocytes (1997)
    Springer
    Pflügers Archiv 434 (1997), S. 505-507 
    Details
    ISSN: 1432-2013
    Keywords: Key words Carbachol ; Nonselective cationic current ; Protein kinase C ; smooth muscle
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract  The effect of protein kinase C (PKC) on carbachol (CCh)-activated nonselective cationic current (I CCh) was investigated in guinea-pig gastric myocytes using a PKC activator, phorbol 12, 13 dibutyrate (PDBu). Pretreatment with 1 μ M PDBu suppressed I CCh by 96.5 ± 2.9% (n = 14) in a reversible manner in nystatin-perforated mode. In the presence of 1 μM chelerythrine , a PKC inhibitor, inhibition of I CChby PDBu was not seen. In whole-cell mode, the inhibition of I CCh by PDBu was dependent on intracellular MgATP. In the presence of MgATP in the pipette, PDBu decreased I CCh by 98.8 ± 1.2% (n = 5) as was observed in nystatin-perforated mode. However, PDBu had little effect on I CCh in the absence of MgATP in the pipette; the extent of inhibition was 12.7 ± 4.3% (n = 8). PDBu also suppressed the generation of cationic current induced by intracellularly perfused GTP[γS]. In the PDBu-pretreated group (n = 9) and PDBu-untreated control group (n = 6), GTP[γS]-induced currents were 6.7 ± 2.4 pA and 236 ± 23 pA, respectively. These results suggest that PKC modulates I CCh at postreceptor sites via protein phosphorylation.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s004240050429
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    Paper (German National Licenses)
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  • 3
    Electronic Resource
    Electronic Resource
    Protein kinase C mediates the desensitization of CCh-activated nonselective cationic current in guinea-pig gastric myocytes (1998)
    Springer
    Pflügers Archiv 436 (1998), S. 1-8 
    Details
    ISSN: 1432-2013
    Keywords: Key words Smooth muscle ; Nonselective cationic current ; Carbachol (CCh) ; Protein kinase C (PKC) ; Desensitization
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract  The possibility of the protein kinase C (PKC) pathway being a mechanism underlying the desensitization of carbachol- (CCh-)activated nonselective cationic current (I CCh) was investigated in a study of guinea-pig gastric myocytes. Using the conventional whole-cell patch-clamp technique with symmetrical CsCl-rich solution in pipette and bath, I CCh was induced by bath application of 50 µM CCh. With 0.5 mM EGTA [ethyleneglycol-bis(β-aminoethyl ether)-N,N,N′,N′-tetraacetic acid] in the pipette solution (0.5 mM [EGTA]i), I CCh decayed spontaneously (desensitization of I CCh) to around 20% within 10 min. Desensitization of I CCh was significantly attenuated with 2 mM [EGTA]i. At a concentration of 20 µM OAG (1-oleoyl-2-acetyl-sn-glycerol), a PKC activator, inhibited I CCh at 0.5 mM [EGTA]i but far less at 2 mM [EGTA]i (18% and 81% of control, respectively). The same cationic current induced by intracellular guanosine-5′-O-(3-thiotriphosphate) (GTP[γ-S]) was not inhibited by OAG with 0.5 mM [EGTA]i. The pretreatment of gastric myocytes with PKC inhibitors, either 1 µM chelerythrine or 1 µM peptide inhibitor, attenuated the desensitization of I CCh. [Ca2+]i was also measured by single cell microfluorometry using fura-2. Under CCh stimulation with 2 mM [EGTA]i, [Ca2+]i did not increase above 100 nM while it increased to around 260 nM with 0.5 mM [EGTA]i. These results suggest that the desensitization of I CCh is partly due to the Ca2+-dependent PKC pathway in guinea-pig gastric myocytes.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s004240050597
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    Paper (German National Licenses)
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  • 4
    Electronic Resource
    Electronic Resource
    Solubilization of hydroxyapatite by Enterobacter agglomerans and cloned Escherichia coli in culture medium (1997)
    Springer
    Biology and fertility of soils 24 (1997), S. 347-352 
    Details
    ISSN: 1432-0789
    Keywords: Key words Phosphate-solubilizing bacteria ; Hydroxyapatite ; Enterobacter agglomerans ; Organic acids ; Phosphate-solubilizing genes ; Rhizosphere ; Wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Geosciences , Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Phosphate-solubilizing bacteria (PSB) possessing the ability to solubilize insoluble inorganic phosphate were isolated from the rhizosphere soil of wheat. A laboratory study was conducted to investigate the solubilization of phosphate by a known PSB, Enterobacter agglomerans, and by a genetically manipulated bacterium, Escherichia coli. A second laboratory study investigated the release of P from E. agglomerans compared with known acids. For the first laboratory study, a cosmid (pHC79) library of phosphate-solubilizing gene(s) from E. agglomerans chromosome DNA was constructed in E. coli JM109. The clone JM109 (pKKY) showing phosphate solubilization properties was screened on standard medium containing hydroxyapatite (HY). The P concentration significantly increased at 5 and 10 days for JM109 (pKKY) compared with JM109 (pHC79), the control. Although the P concentration increased, there was no significant change in their pHs. Furthermore, an increase in colony-forming units (CFUs) was seen at 5 and 10 days for JM109 (pKKY) but not for JM109 (pHC79). Artificial acidification of the culture medium with HCl, citric acid, oxalic acid, and lactic acid was achieved by shaking for 48h. Acidification with these selected acids solubilized more HY than E. agglomerans growing for 42h at similar pHs. However, a high P concentration was measured in culture medium with E. agglomerans growing for 84h despite similar pHs. Our results suggest that acid production may play an important role in HY solubilization, but is not the sole reason for the increase in P concentration in culture medium.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s003740050256
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    Paper (German National Licenses)
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  • 5
    Electronic Resource
    Electronic Resource
    Effect of phosphate-solubilizing bacteria and vesicular-arbuscular mycorrhizae on tomato growth and soil microbial activity (1997)
    Springer
    Biology and fertility of soils 26 (1997), S. 79-87 
    Details
    ISSN: 1432-0789
    Keywords: Key words Organic acids ; Phosphate-solubilizing ; bacteria ; Vesicular-arbuscular mycorrhizae ; Glomus etunicatum ; Enterobacter agglomerans
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Geosciences , Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract The interaction of vesicular-arbuscular mycorrhizae (VAM) and phosphate-solubilizing bacteria (PSB) on plant growth, soil microbial activities, and the production of organic acids was studied in non-sterile soil containing hydroxyapatite and glucose. Glomus etunicatum (VAM), a fungus, and Enterobacter agglomerans, a bacterium able to solubilize insoluble phosphate, were used as inocula. Three treatments and a control were used: inoculation with E. agglomerans (treatment E), inoculation with G. etunicatum (treatment G), inoculation with E. agglomerans+G. etunicatum (treatment E+G) and the control (C). Inoculation with E, G, or E+G had increased plant growth by days 35, 55, and 75 compared with the control. Microbial biomass carbon (C) and alkaline phosphatase activity in the rhizosphere generally increased with time. Alkaline phosphatase activity was higher in treatments G and E+G compared with the control at 35 and 55 days. The highest acid phosphatase activity was observed in treatment E at 35 days; however, this markedly decreased with time. A significantly higher soluble phosphorus (P) concentration was observed in treatments E and E+G on day 55 compared with C. However, there was no significant difference in soluble P concentration in the rhizosphere between treatments with time. The P concentration was greatest in all treatments on day 55. The highest oxalic acid concentration was observed in the rhizosphere of the non-sterile soil in E+G on day 35. Total N and P uptake in plants from treatments E and G were higher compared with the control. However, the highest N and P uptake was observed in treatment E+G. This study suggests a synergistic interaction between E. agglomerans and G. etunicatum.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s003740050347
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    Paper (German National Licenses)
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