ISSN:
1420-908X
Keywords:
Tumour necrosis factorα
;
Interleukin-1β
;
Myeloperoxidase
;
Tissue-chamber (mouse)
;
Inflammation
Source:
Springer Online Journal Archives 1860-2000
Topics:
Medicine
Notes:
Abstract A tissue-chamber model of inflammation in mice has been modified and used to investigate the kinetics of zymosan-induced inflammatory mediators such as tumour necrosis factorα (TNFα), interleukin-1β (IL-1β) and prostaglandin E2 (PGE2). In addition, the influx of polymorphonuclear leukocytes (PMN) into the chamber fluid and the granuloma surrounding the chamber was measured by myeloperoxidase (MPO) activity using a new microtitre plate assay. TNFα and IL-1β reached peak concentrations at 3 and 6 h respectively after zymosan injection. Intermediate high concentrations of IL-1β were observed until the end of the experiment at 72 h, but TNFα concentrations decreased from 24 h to biologically insignificant values. In contrast, exudate PGE2 and MPO activity increased up to 24 h after zymosan injection and remained high until 72 h. At 6h after zymosan challenge, oral pre-treatment with prednisolone (3 to 30mg/kg) dose-dependently reduced TNFα, IL-1β and PGE2 concentrations while indomethacin (0.3 to 3 mg/kg) significantly attenuated PGE2, slightly enhanced TNFα and had no effect on IL-1β concentrations in the exudate. Both drugs had similar potencies against exudate and tissue MPO activities. Prednisolone inhibited IL-1β at 72 h post-zymosan. Indomethacin was more potent than prednisolone against PGE2 (ID50 of 〈0.3 versus 0.6mg/kg). The data obtained confirm the usefulness and reliability of this model in evaluating the effects of anti-inflammatory agents on inflammatory mediators induced by zymosan.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF01796267
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