Library

X
feed icon rss

Your email was sent successfully. Check your inbox.

An error occurred while sending the email. Please try again.

Proceed reservation?

Export
  • 11
    Electronic Resource
    Electronic Resource
    Kongenitale myotone Dystrophie (1997)
    Springer
    Monatsschrift Kinderheilkunde 145 (1997), S. 588-592 
    Details
    ISSN: 1433-0474
    Keywords: Schlüsselwörter Kongenitale myotone Dystrophie ; Curshmann-Steinert-Batten-Syndrom ; CTG-Trinukleotid-Sequenzwiederholung ; Dystrophia myotonica ; Facies myopathica ; Key words Congenital myotonia dystrophy ; Curshmann-Steinert-Batten-Syndrom ; CTG-Trinukleotid sequence repetition ; Distrophy myotonica ; Facies myopathica
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Description / Table of Contents: Summary The subject of this report is a newborn female, suffering from hypotonia and breathing difficulties, delivered in the 37 week with the help of forceps. Because of the nature of her symptons and those of the mother, which included Facies myopathica and Myotonia, the possibility of the expansion of a CTG-Trinukleotidsequence in the area of Chromosom 19 was explored. For this purpose a technique, developed in 1992, for the identification of molecular genetic characteristics was used. Instead of the 5 to 27 copies of the CTG sequence normally found in the population, the sick child had 1000 and the mother more than 700. EMG, in which a classical relases of myotones (Fall-Fight-Bomberscream) were found, confirmed the neurological diagnosis. Discussion: In the course of time the newborn child showed the classical problems of hyptotonia, such as respiratory difficulties, eating disorders leading to a loss of weight, meteroism, and after another stay in hospital, symptoms of Ileus.
    Notes: Zusammenfassung Es wird über ein in der 37. SSW durch Forzeps geborenes Mädchen berichtet, das durch Hypotonie und Ateminsuffizienz auffiel. Diese Symptome, ebenso wie die Facies myopathica und die Myotonie der Hand der Mutter bei der Begrüßung, gaben den Hinweis zur Durchführung der seit 1992 möglichen molekulargenetischen Bestimmung der Expansion einer CTG-Trinukleotidsequenz im Bereich des Chromosoms 19. Statt der in der Normalbevölkerung 5–27 Kopien dieser CTG-Sequenz-Wiederholung fanden sich bei dem erkrankten Kind über 1000 und bei der Mutter über 700. Darüber hinaus konnte durch eine neurologische Untersuchung der Mutter die bisher unbekannte Diagnose bestätigt werden, wobei im EMG eine klassische myotone Entladung (Sturz-Kampf-Bomber-Geräusch) gefunden wurde. Diskussion: Beim Neugeborenen traten im weiteren Verlauf die klassischen Folgeprobleme der Hypotonie wie Respiratorbedürftigkeit und Ernährungsprobleme mit relativer Gewichtsabnahme, Meteorismus und bei einer erneuten stationären Aufnahme Ileussymptomatik auf.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s001120050156
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 12
    Electronic Resource
    Electronic Resource
    Structural and biochemical properties of glycosylated and deglycosylated glucose oxidase from Penicillium amagasakiense (1997)
    Springer
    Applied microbiology and biotechnology 47 (1997), S. 502-507 
    Details
    ISSN: 1432-0614
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Abstract Glucose oxidase from Penicillium amagasakiense was purified to homogeneity by ion-exchange chromatography and deglycosylated with endoglycosidase H. On the basis of gas chromatography and sodium dodecyl sulphate/polyacrylamide gel electrophoretic (SDS-PAGE) analyses, the protein-bound high-mannose-type carbohydrate moiety corresponded to 13% of the molecular mass of glycosylated glucose oxidase. A total of six N-glycosylation sites per dimer were determined from the N-acetylglucosamine content. The enzymatically deglycosylated enzyme contained less than 5% of the original carbohydrate moiety. A molecular mass of 130 kDa (gel filtration) and 133 kDa (native PAGE) was determined for the dimer and 67 kDa (SDS-PAGE) for the monomer of the deglycosylated enzyme. The N-terminal sequence, which has not been published for glucose oxidase from P. amagasakiense to date and which showed less than 50% homology to the N terminus of glucose oxidase from Aspergillus niger, and the amino acid composition were not altered by the deglycosylation. Deglycosylation also did not affect the kinetics of glucose oxidation or the pH and temperature optima. It also did not increase the susceptibility of the enzyme to proteolytic degradation. However, deglycosylated glucose oxidase exhibited decreased pH and thermal stability. The thermal stability of both enzymes was shown to be dependent on the buffer concentration and was enhanced by certain additives, particularly 1 M (NH4)2SO4, which stabilised glucose oxidase 100- to 300-fold at 50 °C and pH 7–8, and 2 M KF, which stabilised the enzyme up to 36-fold at 60 °C and pH 6. In sodium acetate buffer, changes in pH (4–6) affected the affinity for glucose but had no effect on the V max of the reaction. In contrast, in TRIS buffer, pH 8, a 10-fold decrease in V max and a 2-fold decrease in K m were observed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s002530050963
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 13
    Electronic Resource
    Electronic Resource
    High-level expression of the thermoalkalophilic lipase from Bacillus thermocatenulatus in Escherichia coli (1998)
    Springer
    Applied microbiology and biotechnology 49 (1998), S. 405-410 
    Details
    ISSN: 1432-0614
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Abstract An efficient expression system for the previously only weakly expressed thermophilic lipase BTL2 (Bacillus thermocatenulatus lipase 2) was developed for the production of large amounts of lipase in Escherichia coli. Therefore, the gene was subcloned in the pCYTEXP1 (pT1) expression vector downstream of the temperature-inducible λ promoter PL. Three different expression vectors were constructed: (i) pT1-BTL2 containing the mature lipase gene, (ii) pT1-preBTL2 containing the prelipase gene and (iii) pT1-OmpABTL2 containing the mature lipase gene fused to the signal peptide of the OmpA protein, the major outer membrane protein of E. coli. With pT1-BTL2 and pT1-preBTL2, comparable expression levels of 7000–9000 U/g cells were obtained independently of the E. coli host. In contrast, with E. coli JM105 harbouring pT1-OmpABTL2, 660 000 soluble lipase U/g cells was produced, whereas, with E. coli DH5α and BL321, production levels of 30 000 U/g cells were achieved. However, most of the lipase remained insoluble but active after cell breakage because of the unprocessed OmpA signal peptide. A simple cholate extraction followed by proteinase K cleavage and ultrafiltration allowed the isolation of 1.15 × 106 units of 90% pure mature lipase/wet cells.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s002530051190
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 14
    Electronic Resource
    Electronic Resource
    Disposable sensor for measuring the biochemical oxygen demand for nitrification and inhibition of nitrification in wastewater (1999)
    Springer
    Applied microbiology and biotechnology 51 (1999), S. 112-117 
    Details
    ISSN: 1432-0614
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Abstract A disposable-type microbial sensor was developed for the determination of both the biochemical oxygen demand for nitrification (N-BOD) and inhibiting effects on nitrifying bacteria. The sensor was based on the respiratory activity of nitrifying bacteria immobilized on a miniature oxygen electrode. Typical response times for measuring N-BOD of ammonium standard solutions as well as of wastewater samples were in the range of 6–12 min. A dynamic evaluation of the signals after a measuring time of 120 s also resulted in good reproducibility and sensitivity. A daily profile of a municipal sewage plant was recorded, comparing the biosensor data with two standard methods. For the measurement of nitrification-inhibiting effects a 120-s dynamic signal evaluation was preferred to a steady-state method because of the long recovery times resulting from extended exposure to inhibitors. However, steady-state measurement techniques allowed allylthiourea detection with a ten times higher sensitivity. Because of the advantages of this miniaturized electrode, e.g. short response time, simple measuring procedure and low costs of production, this sensor system is considered to be suitable for commercial application in environmental analysis.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s002530051371
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 15
    Electronic Resource
    Electronic Resource
    Purification and some properties of a novel microbial lactate oxidase (1996)
    Springer
    Applied microbiology and biotechnology 45 (1996), S. 600-606 
    Details
    ISSN: 1432-0614
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Abstract  Geotrichum candidum was found to produce a lactate oxidase. The enzyme was purified by gel filtration and ion-exchange chromatography. The purified lactate oxidase showed a molecular mass of 50 kDa under denaturing and about 400 kDa under non-denaturing conditions. Transmission electron micro-scopy analysis confirmed an octameric structure. FMN was found to be a cofactor for this enzyme. Polarographic studies confirmed an oxygen uptake by the lactate oxidase. The enzyme showed specificity towards the L isomer of lactate and did not oxidise pyruvate, fumarate, succinate, maleate and ascorbate. It was stable at alkaline pH and also for 15 min at 45°C. The addition of glycerol and dextran 500 000 to the enzyme sample enhanced storage stability.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s002530050736
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 16
    Electronic Resource
    Electronic Resource
    [Ru(η5-C5H5)(η4-C5H4O){As(CH3)3}]PF6 and [Ru(η5-C5H5)(η4-C5H4O){As(C6H5)3}]PF6 (1995)
    Oxford [u.a.] : International Union of Crystallography (IUCr)
    Acta crystallographica 51 (1995), S. 361-364 
    Details
    ISSN: 1600-5759
    Source: Crystallography Journals Online : IUCR Backfile Archive 1948-2001
    Topics: Chemistry and Pharmacology , Geosciences , Physics
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1107/S0108270194005688
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 17
    Electronic Resource
    Electronic Resource
    Low free-stream turbulence in test sections through packed beds and fibrous mats (1999)
    Springer
    Experiments in fluids 26 (1999), S. 451-459 
    Details
    ISSN: 1432-1114
    Source: Springer Online Journal Archives 1860-2000
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
    Notes: Abstract  At low speeds the free-stream turbulence in test-sections of water channels and wind tunnels can hardly be suppressed with a conventional set of screens in the settling chamber. In many cases the friction of screens is not high enough to quench the turbulence created by compressors, orifices, bends, diffusers, and nozzles. Without creating new turbulence the kinetic energy of already existing turbulence can only be eliminated by laminar friction somewhere upstream of the test-section. This laminar friction can be provided by packed beds and fibrous mats. Due to the small size of pellets or strands and due to the low speed in the settling chamber the corresponding Reynolds number may be low enough to achieve laminar or near laminar flow. An additional bonus may be obtained from the suppression of flow separation in the diffuser, if the packed bed or the fibrous mats are arranged downstream of the diffuser with a minimum required drag, whereas the drag of screens would be below this required minimum. The used water channel and the wind tunnel were not custom-made to the specifications for low free-stream turbulence. Even though, it was possible to get the free stream turbulence down to 0.063% with a packed bed and to 0.037% with fibrous mats. However, in comparison with the fibrous mats, the drag coefficient of the packed bed was almost ten times higher.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s003480050309
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 18
    Electronic Resource
    Electronic Resource
    Development of a dipstick immunoassay for quantitative determination of 2,4-dichlorophenoxyacetic acid in water, fruit and urine samples (1999)
    Springer
    Fresenius' journal of analytical chemistry 364 (1999), S. 584-589 
    Details
    ISSN: 1432-1130
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Abstract A sensitive dipstick assay for 2,4-dichlorophenoxyacetic acid (2,4-D) detection was developed. The assay was based on the competitive reaction of 2,4-D and enzyme tracer with monoclonal antibodies immobilised on an Ultrabind® membrane. The binding of enzyme tracer on the test strip was determined by a simple, portable reflectometer as remission at 657 nm. Using this technique, 2,4-D could be detected in a concentration range of 0.5 μg/L to 100 μg/L. The center point of the 2,4-D test was found at a concentration of 6 μg/L. Cross-reactivity with 2,4,5-trichlorophenoxyacetic acid (2,4,5-T) as determined by this dipstick assay was 2.5% and 3% by standard ELISA technique using microtiter plates. The assay was applied in the detection of 2,4-D in real water samples, and sensitivity was comparable to spiked water samples. If combined with an effective extraction procedure that results in recovery rates of 90%, the dipstick assay can be used to monitor human exposure to 2,4-D from contamination in water, from oranges and in testing urine samples.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s002160051390
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 19
    Electronic Resource
    Electronic Resource
    New anastomosis technique for (laparoscopic) instrumental small-diameter anastomosis (1995)
    Springer
    Surgical endoscopy and other interventional techniques 9 (1995), S. 444-449 
    Details
    ISSN: 1432-2218
    Keywords: Surgical instrumentation ; Biliary surgery ; Anastomosing technology ; Malignant jaundice
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract This study presents a new technique for visceral anastomosis. The principle consists of connecting the two parts to be anastomosed around a reabsorbable stent which is transluminally introduced into a small-diameter viscus, where it is fixed. Advancing a larger tube along the axis of the machine, the larger, perforated viscus is inverted and pulled over the stent, and finally a rubber band pops off the machine endoluminally in order to fix the intestinal walls in seroserosal contact onto the stent. To evaluate this “micro” anastomosis, a biliary bypass (choledochojejunostomy and roux-en-y-loop) was performed in ten pigs. Nine of ten animals showed biliary bypass with good runoff in contrast radiography and completely reabsorbed stent after a 3-month follow-up. Weight gain, bilirubin, and alkaline phosphatase were normal. This technology demonstrates a safe and quick way to perform instrumental “micro” anastomosis without remnant foreign material.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00187172
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 20
    Electronic Resource
    Electronic Resource
    Regulation of σ B-dependent transcription of sigB and asp23 in two different Staphylococcus aureus strains (1999)
    Springer
    Molecular genetics and genomics 261 (1999), S. 558-566 
    Details
    ISSN: 1617-4623
    Keywords: Key wordssigB ; asp23 ; rsbU ; Staphylococcus aureus ; Bacillus subtilis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The alkaline shock protein Asp23 was identified as a σ B-dependent protein in Staphylococcus aureus. In Bacillus subtilis, the asp23 promoter from S. aureus is regulated like other σ B-dependent promoters, which are strongly induced by heat and ethanol stress. However, almost no induction of asp23 expression was found after heat or ethanol stress in S. aureus MA13 grown in a synthetic medium, where the basal expression level of asp23 is high. Under the same experimental conditions the σ B gene itself showed a similar expression pattern: it was highly expressed in synthetic medium but not induced by heat or ethanol stress. In contrast, σ B activity was increased by heat stress when the cells were grown in a complex medium. The constitutive expression of sigB and σ B-dependent stress genes in S. aureus MA13 grown in a synthetic medium is in a sharp contrast to the regulation of σ B activity in B. subtilis, and needs further investigation. A deletion of 11 bp in the rsbU gene, which encodes the phosphatase that acts on RsbV (the anti-anti-sigma factor), in S. aureus NCTC 8325-4 might be responsible for the failure of heat stress to activate σ B in complex medium, and thus reduce the initiation of transcription at σ B-dependent promoters in this strain.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s004380051001
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
Close ⊗
This website uses cookies and the analysis tool Matomo. More information can be found here...