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  • Articles: DFG German National Licenses  (4)
  • 1995-1999  (4)
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  • Articles: DFG German National Licenses  (4)
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  • 1
    Electronic Resource
    Electronic Resource
    Identification of five physiological races of blackspot, Diplocarpon rosae, Wolf on roses (1998)
    Oxford, UK : Blackwell Publishing Ltd
    Plant breeding 117 (1998), S. 0 
    Details
    ISSN: 1439-0523
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Differentiation into physiological races of Diplocarpon rosae, which causes blackspot on wild and cultivated roses, was investigated with single conidial isolates of the pathogen. Infection experiments with a simple excised leaf assay demonstrated differential interactions between single isolates and a set of 10 test rose genotypes. Differential reactions could be observed among rose varieties as well as between and within a wild rose species. Accordingly, five different physiological races can be identified among the 15 isolates tested. A breeding line and one genotype of the wild species Rosa wichuraiana were found to be resistant against all isolates tested. These data will be employed in further investigations on the genetics of blackspot resistance in roses and for resistance breeding programmes.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1439-0523.1998.tb01937.x
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Effective pairwise combination of long primers for RAPD analyses in roses (1998)
    Oxford, UK : Blackwell Publishing Ltd
    Plant breeding 117 (1998), S. 0 
    Details
    ISSN: 1439-0523
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Twenty-four primers of different lengths (eight each of 10, 15 and 20 bp) were tested each in RAPD reactions with DNA of Rosa multiflora and Rosa canina. The reactions with single primers of a particular length were compared with all 28 pairwise combinations within each length class in both single primer reactions and in primer combination reactions. All primer classes produced fragment patterns of comparable complexity. However, the number of new fragment patterns and the number of fragments containing repetitive DNA was dependent on the primer length. Combinations of long 15- and 20-mer primers produced more new fragments and a lower amount of repetitive DNA than shorter 10-mer primers. Implications for the use of long primer combinations in projects which require large marker numbers are discussed in comparison with other marker systems.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1439-0523.1998.tb01469.x
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    Paper (German National Licenses)
    Fulltext
  • 3
    Electronic Resource
    Electronic Resource
    Construction of a genetic linkage map for roses using RAPD and AFLP markers (1999)
    Springer
    Theoretical and applied genetics 99 (1999), S. 891-899 
    Details
    ISSN: 1432-2242
    Keywords: Key words Rosa ; Genetic mapping ; Molecular markers ; Flower colour ; Petal number
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  A segregating population of diploid rose hybrids (2n = 2x = 14) was used to construct the first linkage maps of the rose genome. A total of 305 RAPD and AFLP markers were analysed in a population of 60 F1 plants based on a so-called ”double-pseudotestcross” design. Of these markers 278 could be located on the 14 linkage groups of the two maps, covering total map lengths of 326 and 370 cM, respectively. The average distances between markers in the maps for 93/1–117 and 93/1–119 is 2.4 and 2.6 cM, respectively. In addition to the molecular markers, genes controlling two phenotypic characters, petal number (double versus single flowers) and flower colour (pink versus white), were mapped on linkage groups 3 and 2, respectively. The markers closest to the gene for double flowers, Blfo, and to the gene for pink flower colour, Blfa, cosegregated without recombinants. The maps provide a tool for further genetic analyses of horticulturally important genes as, for example, resistance genes and a starting point for marker-assisted breeding in roses.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s001220051310
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    Paper (German National Licenses)
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  • 4
    Electronic Resource
    Electronic Resource
    Genetic analysis of resistance to blackspot (Diplocarpon rosae) in tetraploid roses (1998)
    Springer
    Theoretical and applied genetics 96 (1998), S. 228-231 
    Details
    ISSN: 1432-2242
    Keywords: Key words Diplocarpon rosae ; Blackspot ; Rosa ; Resistance ; Genetic analysis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract   Diplocarpon rosae is the causal agent of rose blackspot, one of the most severe diseases of field-grown roses. The genetics of resistance to this pathogen was investigated in crosses between tetraploid rose genotypes. The hybrid breeding line 91/100-5, which exhibits a broad resistance to all isolates tested so far, was selfed to produce an F2 population, backcrossed to the susceptible tetraploid variety ‘Caramba’ and crossed to the susceptible varieties ‘Heckenzauber’, ‘Pariser Charme’ and ‘Elina’. Infection experiments resulted in segregation ratios consistent with the presence of a single dominant resistance locus in the duplex configuration in the hybrid 91/100-5. This suggests, together with previous data on the race structure of the fungus, a “gene-for-gene” type of interaction in the pathosystem Diplocarpon/Rosa. We propose to designate this gene Rdr1, which is the first resistance gene described in the genus Rosa. The advantages and limitations of such an interaction type for future rose breeding programmes and for marker-assisted selection strategies are discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s001220050731
    Library Location Call Number Volume/Issue/Year Availability
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    Paper (German National Licenses)
    Fulltext
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