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  • Articles: DFG German National Licenses  (6)
  • 1995-1999  (6)
Source
  • Articles: DFG German National Licenses  (6)
Material
Years
Year
Keywords
  • 1
    Electronic Resource
    Electronic Resource
    Thermostable d-hydantoinase from thermophilic Bacillus stearothermophilus SD-1: characteristics of purified enzyme (1995)
    Springer
    Applied microbiology and biotechnology 43 (1995), S. 270-276 
    Details
    ISSN: 1432-0614
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Abstract One thousand thermophiles isolated from soils were screened for hydantoinase and its thermostability. One thermophilic bacterium that showed the highest thermostability and activity of hydantoinase was identified to be Bacillus stearothermophilus SD-1 according to morphological and physiological characteristics. The hydantoinase of B. stearothermophilus SD-1 was purified to homogeneity via ammonium sulfate fractionation, anion-exchange chromatography, heat treatment, hydrophobic-interaction chromatography, and preparative gel electrophoresis. The relative molecular mass of the hydantoinase was determined to be 126 kDa by gel-filtration chromatography, and a value of 54 kDa was obtained as a molecular mass of the subunit on analytical sodiumdodecylsulfate/polyacrylamide gel electrophoresis. The hydantoinase was strictly d-specific and metal-dependent. The optimal pH and temperature were about 8.0 and 65°C respectively, and the half-life of the d-hydantoinase was estimated to be 30 min at 80°C, indicating the most thermostable enzyme so far.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00172823
    Library Location Call Number Volume/Issue/Year Availability
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    Paper (German National Licenses)
    Fulltext
  • 2
    Electronic Resource
    Electronic Resource
    Thermostable d-hydantoinase from thermophilic Bacillus stearothermophilus SD-1: characteristics of purified enzyme (1995)
    Springer
    Applied microbiology and biotechnology 43 (1995), S. 270-276 
    Details
    ISSN: 1432-0614
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Abstract  One thousand thermophiles isolated from soils were screened for hydantoinase and its thermostability. One thermophilic bacterium that showed the highest thermostability and activity of hydantoinase was identified to be Bacillus stearothermophilus SD-1 according to morphological and physiological characteristics. The hydantoinase of B. stearothermophilus SD-1 was purified to homogeneity via ammonium sulfate fractionation, anion-exchange chromatography, heat treatment, hydrophobic-interaction chromatography and preparative gel electrophoresis. The relative molecular mass of the hydantoinase was determined to be 126 kDa by gel-filtration chromatography, and a value of 54 kDa was obtained as a molecular mass of the subunit on analytical sodiumdodecylsulfate/polyacrylamide gel electrophoresis. The hydantoinase was strictly d-specific and metal-dependent. The optimal pH and temperature were about 8.0 and 65°  C respectively, and the half-life of the d-hydantoinase was estimated to be 30 min at 80°  C, indicating the most thermostable enzyme so far.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s002530050401
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    Paper (German National Licenses)
    Fulltext
  • 3
    Electronic Resource
    Electronic Resource
    Study of siloxane-modified epoxy resin using thermally stimulated current(II) (1997)
    Springer
    Polymer bulletin 39 (1997), S. 193-199 
    Details
    ISSN: 1436-2449
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology , Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics , Physics
    Notes: Abstract. Thermally stimulated current (TSC) and relaxation map analysis (RMA) was used to characterize the low temperature relaxation of epoxy resin modified with siloxane oligomers. In aminopropyl-terminated siloxane oligomer (ATSO) the β-relaxation of epoxy resin and the glass transition temperature of siloxane oligomer were folded regardless of the concentration of diphenyl. The β-relaxation of epoxy resin and the glass transition temperature of oxiranylmethoxy-terminated siloxane oligomer (OTSO) were folded and shifted to higher temperature as the concentration of diphenyl in siloxane oligomer increased. In the systems containing of diphenyl in siloxane oligomer a new relaxation peak due to the space charge was observed in the range of − 80 °C to − 50 °C and − 30 °C to 5 °C. As the concentration of diphenyl increased the compensation temperature (Tc) and the degree-of-disorder (DOD) were increased while the compensation time, τ c was decreased.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s002890050138
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    Paper (German National Licenses)
    Fulltext
  • 4
    Electronic Resource
    Electronic Resource
    SAN-Na+-montmorillonite nanocomposite for electrorheological material (1999)
    Springer
    Journal of materials science 18 (1999), S. 1505-1507 
    Details
    ISSN: 1573-4811
    Source: Springer Online Journal Archives 1860-2000
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1006642317328
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    Paper (German National Licenses)
    Fulltext
  • 5
    Electronic Resource
    Electronic Resource
    Primary Structure, sequence analysis, and expression of the thermostable d - hydantoinase from Bacillus stearothermophilus SD1 (1997)
    Springer
    Molecular genetics and genomics 255 (1997), S. 152-156 
    Details
    ISSN: 1617-4623
    Keywords: Key words D-Hydantoinase ; Amidohydrolase ; Bacillus stearothermophilus SD1
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The gene coding for the thermostable d-hydantoinase from the thermophilic bacterium Bacillus stearothermophilus SD1 was cloned and its nucleotide sequence was completely determined. The d-hydantoinase protein showed considerable amino acid sequence homology (20–28%) with other hydantoinases and functionally related allantoinases and dihydroorotases. Strikingly the sequence of the enzyme from B. stearothermophilus SD1 exhibited greater than 89% identity with hydantoinases from thermophilic bacteria. Despite the extremely high amino acid homology among the hydantoinases from thermophiles, the C-terminal regions of the enzymes were completely different in both sequence and predicted secondary structure, implying that the C-terminal region plays an important role in determining the biochemical properties of the enzymes. Alignment of the sequence of the d-hydantoinase from B. stearothermophilus SD1 with those of other functionally related enzymes revealed four conserved regions, and five histidines and an acidic residue were found to be conserved, suggesting a close evolutionary relationship between all these enzymes.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/PL00008610
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    Paper (German National Licenses)
    Fulltext
  • 6
    Electronic Resource
    Electronic Resource
    Study of rubber-modified epoxy resin using thermally stimulated current (1998)
    Springer
    Polymer bulletin 40 (1998), S. 599-605 
    Details
    ISSN: 1436-2449
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology , Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics , Physics
    Notes: Summary In order to characterize the low temperature relaxation of epoxy resin modified with amine-terminated butadiene acrylonitrile copolymers (ATBN), thermally stimulated current (TSC) and relaxation map analysis (RMA) were investigated. Four relaxation peaks at around −140, −100, −60 and 0°C were observed as the indication of γ-, β-relaxation of epoxy resin, Tg, new unknown peak of ATBN, respectively. The unknown peak at around 0°C was observed due to dipole orientation. The RMA data was showed that compensation temperature (Tc) and degree-of-disorder (DOD) were increased with increasing the content of acrylonitrile and ATBN, whereas the compensation time (τc) was decreased. It can be concluded that the cooperative molecular motion in cured epoxy resin was more active as the concentration of acrylonitrile and ATBN content increases.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s002890050296
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    Paper (German National Licenses)
    Fulltext
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