ISSN:
1573-5028
Keywords:
Arachis hypogaea
;
Botrytis cinerea
;
chitinase
;
fungal elicitior
;
ethylene
;
salicylate
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract Two different genes encoding class II chitinases from peanut (Arachis hypogaea L. cv. NC4), A.h.Chi2;1 and A.h.Chi2;2, have been cloned. In peanut cell suspension cultures, mRNA levels of A.h.Chi2;2 increased after ethylene or salicylate treatment and in the presence of conidia from Botrytis cinerea. The second gene, A.h.Chi2;1, was only expressed after treatment with the fungal spores. Transgenic tobacco plants containing the complete peanut A.h.Chi2;1 gene exhibited essentially the same expression pattern in leaves as observed in peanut cell cultures. Expression characteristics of transgenic tobacco carrying a promoter-GUS fusion of A.h.Chi2;1 are described.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00020121
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