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  • Artikel: DFG Deutsche Nationallizenzen  (5)
  • 1995-1999  (5)
Datenquelle
  • Artikel: DFG Deutsche Nationallizenzen  (5)
Materialart
Erscheinungszeitraum
Jahr
  • 1
    Digitale Medien
    Digitale Medien
    Springer
    Applied microbiology and biotechnology 43 (1995), S. 887-892 
    ISSN: 1432-0614
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Werkstoffwissenschaften, Fertigungsverfahren, Fertigung
    Notizen: Abstract  A resting-cell assay was established to evaluate the cholesterol reductase activity of Eubacterium coprostanoligenes ATCC 51222. Cell suspensions from cholesterol-free media rapidly reduced cholesterol to coprostanol. Optimal assay conditions in a 1-ml reaction mixture were determined to be up to 1 h of incubation and up to 0.25 mg bacterial protein/assay with at least 1 mM cholesterol as substrate. The cholesterol reductase activity in cells decreased as a function of storage time at 22°C, 4°C and −20°C. Filling the headspace of the reaction mixture with H2 increased the activity about 20%. Optimal cholesterol reductase activity occurred at pH 7.5 in sodium phosphate buffer. Pyruvate and reducing agents in the buffer increased the activity. This study has validated assay conditions for determination of cholesterol reductase activity in resting cells of E. coprostanoligenes.
    Materialart: Digitale Medien
    Bibliothek Standort Signatur Band/Heft/Jahr Verfügbarkeit
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  • 2
    Digitale Medien
    Digitale Medien
    Springer
    Applied microbiology and biotechnology 43 (1995), S. 887-892 
    ISSN: 1432-0614
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Werkstoffwissenschaften, Fertigungsverfahren, Fertigung
    Notizen: Abstract A resting-cell assay was established to evaluate the cholesterol reductase activity ofEubacterium coprostanoligenes ATCC 51222. Cell suspensions from cholesterol-free media rapidly reduced cholesterol to coprostanol. Optimal assay conditions in a 1-ml reaction mixture were determined to be up to 1 h of incubation and up to 0.25 mg bacterial protein/assay with at least 1 mM cholesterol as substrate. The cholesterol reductase activity in cells decreased as a function of storage time at 22°C, 4°C and −20°C. Filling the headspace of the reaction micture with H2 increased the activity about 20%. Optimal cholesterol reductase activity occurred at pH 7.5 in sodium phosphate buffer. Pyruvate and reducing agents in the buffer increased the activity. This study has validated assay conditions for determination of cholesterol reductase activity in resting cells ofE. coprostanoligenes.
    Materialart: Digitale Medien
    Bibliothek Standort Signatur Band/Heft/Jahr Verfügbarkeit
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  • 3
    ISSN: 1432-1793
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract Nyctiphanes australis contained, on a dry weight basis, an average of 52% crude protein and 5.0 to 9.5% lipid. The fatty acid profile of N. australis was markedly unsaturated, with a mean total ω3 fatty acid content of 48.6±2.4% of total fatty acids. N. australis contained high levels of the essential long-chain polyunsaturated fatty acids eicosapentaenoic (EPA, 20:5ω3) and docosahexaencic (DHA, 22:6ω3), ranging from 16.6 to 36.5% and 11.1 to 24.8%, respectively. The concentration of total carotenoids ranged from 137 to 302 μg g−1 dry wt, with no significant differences in concentrations found with season or life stage. The carotenoids were comprised of 79.5% astaxanthin and 20.5% canthaxanthin. The lipid and pigment compositions of N. australis suggest that the species could serve as a suitable feed source for cultured salmonids. Like other euphausiids, N. australis contained high levels of fluoride, with a seasonal range between 277 and 3507 μg g−1 dry wt. The high fluoride levels found in N. australis would not detract from its potential as a feed source for salmonids because ingested fluoride is largely absorbed by the skeleton.
    Materialart: Digitale Medien
    Bibliothek Standort Signatur Band/Heft/Jahr Verfügbarkeit
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  • 4
    ISSN: 1432-1793
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract Midwater fishes were sampled at night to a depth of 400 m in oceanic waters east of Tasmania, Australia, in May/June of 1992, 1993 and 1994. We examined whether there were differences in the distribution and density of these fishes in relation to the subtropical convergence (STC) separating East Australia Current (EAC) water from subantarctic water (SAW) to the south. A total of 23999 fishes from 107 taxa and 43 families were identified. Four new records were identified from the study area. Myctophids and stomiatoids were the main fish taxa captured. We found no evidence of increased density of the combined fish catch in any particular area. However, differences between areas in the density of individual species were noted, although no species was confined to any one area. Ceratoscopelus warmingi, Lobianchia dofleini and Vinciguerria spp. were most abundant in the EAC, whereas Lampichthys procerus was most abundant in the SAW. Only Diaphus danae was found in significantly higher numbers in the STC. Multivariate analysis revealed that community structure in the EAC was significantly different from that of the SAW but not from the STC. Further, when separated by depth, the shallow EAC group was significantly different in all group comparisons bar that with the shallow STC. Our data indicated that the thin EAC layer above 200 m could be distinguished by its fauna from the SAW. However, we could not detect a separate community in the STC. We conclude, therefore, that the STC is not an area of increased micronekton abundance, nor does it contain a distinct community, during the Australian autumn/winter.
    Materialart: Digitale Medien
    Bibliothek Standort Signatur Band/Heft/Jahr Verfügbarkeit
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  • 5
    Digitale Medien
    Digitale Medien
    Springer
    Skeletal radiology 26 (1997), S. 134-136 
    ISSN: 1432-2161
    Schlagwort(e): Key words Elbow ; Dislocation ; Hill-Sachs ; Bankart ; Humerus ; Trauma
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Medizin
    Notizen: Abstract  We describe a new plain radiographic finding in posterior elbow dislocation: a defect in the nonarticular posterior surface of the capitellum caused by impaction of the radial head during dislocation. The defect was visible on a lateral view unobscured by overlying bony structures in the unreduced state and confirmed on both an axial view after reduction and by magnetic resonance imaging. We propose that this lesion is analogous to the Hill-Sachs/ Bankart lesion complex seen in translational glenohumeral injury.
    Materialart: Digitale Medien
    Bibliothek Standort Signatur Band/Heft/Jahr Verfügbarkeit
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