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  • Articles: DFG German National Licenses  (2)
  • 1990-1994  (1)
  • 1985-1989  (1)
  • Blutspuren, Immunoblottierung  (1)
  • Caesarean section scar  (1)
  • 1
    Electronic Resource
    Electronic Resource
    Application of immunoblotting to serum protein phenotyping with reference to α2HS-glycoprotein (AHS) typing of bloodstains (1985)
    Springer
    International journal of legal medicine 95 (1985), S. 153-158 
    Details
    ISSN: 1437-1596
    Keywords: Serum groups, immunoblotting ; α2HS-glycoprotein typing ; Bloodstains, immunoblotting ; Serumgruppen, Immunoblottierung ; α2HS-Glykoprotein, Typisierung ; Blutspuren, Immunoblottierung
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine , Law
    Description / Table of Contents: Zusammenfassung Seren und Blutextrakte wurden auf Polyacrylamidgelen isoelektrisch fokussiert. Die fokussierten Proteine wurden auf Nitrozellulosemembranen durch Diffusion oder elektrophoretisch transferiert. Durch ein spezifisches Antiserum erfolgte auf den Membranen die AG-AK-Reaktion. Nach Waschen der Membranen wurde der gebundene Antikörper mit einem enzymisch markierten Anti-Kaninchen IgG-Antikörper nachgewiesen. Mit dieser Technik konnten die gruppenspezifische Komponente, das α2HS-Glykoprotein, die Komplementkomponenten C6 und C7, der Gerinnungsfaktor 13B und das Plasminogen sehr empfindlich phänotypisiert werden. Vorzugsweise konnten bis zu 6 Monate alte Blutspuren richtig typisiert werden.
    Notes: Summary Sera and bloodstain extracts were subjected to isoelectric focusing in polyacrylamide gels. The focused proteins were transferred to nitrocellulose membranes by diffusion or electrophoretically, then allowed to react with specific antiserum and, after washing, with peroxidase-labeled anti-rabbit IgG. The immune complexes formed on the membranes were detected with 4-chloro-1-naphthol and hydrogen peroxide. Serum group-specific component, α2HS-glycoprotein, the sixth and the seventh component of complement, factor 13B, and plasminogen could be phenotyped with high sensitivity. Bloodstains as old as 6 months could be correctly typed for α2HS-glycoprotein by the blotting technique.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00201072
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Ultrasound examination of caesarean section scars during pregnancy (1991)
    Springer
    Archives of gynecology and obstetrics 248 (1991), S. 129-138 
    Details
    ISSN: 1432-0711
    Keywords: Ultrasound ; Caesarean section scar ; Conventional method ; New method ; Lower uterine segment
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Two hundred and sixteen transverse caesarean section scars were examined sonographically near term by a conventional method (175 scars) and a new method (41 scars). The new method consisted of obtaining a transabdominal longitudinal scan by the conventional method and also by a 3M conductor, a transabdominal frontal scan to give a surface view of the scar, and transperineal and transvaginal longitudinal scans. The new method was used from 16 weeks of gestation onwards. Of 41 scars scanned by the new method, 31 showed good healing, being more than 2 mm in thickness throughout; 10 scars showed poor healing with a thickness of less than 2 mm and loss of continuity. Of 31 patients with good healing, 8 delivered vaginally and the remaining 23 patients had repeat caesarean sections for other obstetric indications. All patients with ultrasound evidence of poor healing had repeat caesarean sections. At operation the thickness of the lower uterine segment was measured with ophthalmic calipers. There were 4 false negative results (4/83: 4.8%) and 1 false positive result (1/43: 2.3%) with conventional ultrasound and no false positives or false negatives with the new method.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02390090
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    Paper (German National Licenses)
    Fulltext
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