ISSN:
1420-9071
Keywords:
Corpora allata
;
juvenile hormone biosynthesis
;
allatostatins
;
phosphoramidon
;
enkephalinase
;
cockroach
;
Diploptera punctata
;
ELISA
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
,
Medicine
Notes:
Abstract Use of the enkephalinase inhibitor phosphoramidon in the in vitro radiochemical assay for juvenile hormone biosynthesis enhanced allatostatin-mediated inhibition of hormone production by corpora allata of the cockroach,Diploptera punctata. Significant increases in inhibition in day 2 virgin female CA by AST 1 (at 10−7 M) and AST 4 (10−8–10−7 M) were observed in the presence of phosphoramidon (10−5M or greater). No significant increases in inhibition were seen in CA from day 6 mated females with AST 4 (10−9–10−7M) and phosphoramidon combined. Phosphoramidon alone had no effect on JH biosynthesis. Analysis of allatostatin content of the CA, as determined by ELISA, revealed that addition of phosphoramidon to the medium increased the endogenous allatostatin conten in CA of virgin and mated females. The similarity in primary structure between allatostatins and enkephalin-like peptides and their similar distribution makes it probable that phosphoramidon acts by preventing breakdown of allatostatins within the CA.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF02124297
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