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  • Articles: DFG German National Licenses  (2)
  • 1990-1994  (2)
  • 1960-1964
  • 1920-1924
  • transport  (2)
  • 1
    Electronic Resource
    Electronic Resource
    Membrane vesicles from brown adipose tissue: A tool for the study of amino acid transport. The case of L-alanine (1992)
    Springer
    Bioscience reports 12 (1992), S. 115-122 
    Details
    ISSN: 1573-4935
    Keywords: alanine ; brown adipose membrane vesicles ; transport
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract A density gradient method is used to isolate membrane vesicles from brown adipose tissue. These respond to changes in osmolarity and show the classical overshoot pattern when L-alanine uptake is assayed. Transport is shown to be effected by two components: a linear (Kd=0.498 min−1) and Na+-dependent saturable component (Km=2.3 mM) and a Vmax=19.9 pmol/μg protein·min). This pattern is similar to that shown by cells isolated from brown adipose tissue.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02351216
    Library Location Call Number Volume/Issue/Year Availability
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    L-alanine transport in isolated cells of interscapular brown adipose tissue in rat (1991)
    Springer
    Bioscience reports 11 (1991), S. 65-71 
    Details
    ISSN: 1573-4935
    Keywords: alanine ; brown adipose cells ; transport
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract The pattern of L-alanine uptake in isolated cells of interscapular brown adipose tissue has been determined. The uptake can be divided into the diffusion component (Kd=0.55 min−1) and a saturable Na+-dependent transport (K M =0.87 mM andV max=155 nmol/min/106 cells). The saturable component can be subdivided into MeAIB-sensitive (K M =1.63 mM andV max=162 nmol/min/106 cells) and MeAIB-insensitive (K M =3.2 mM andV max=39.5 nmol/min/106 cells). This kinetic pattern could indicate the presence of transport system (s) that resemble the commonly described transport systems for alanine uptake in several tissues.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01119193
    Library Location Call Number Volume/Issue/Year Availability
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    Paper (German National Licenses)
    Fulltext
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