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  • Articles: DFG German National Licenses  (8)
  • 1990-1994  (8)
  • 1
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    Annals of the New York Academy of Sciences 656 (1992), S. 0 
    ISSN: 1749-6632
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Natural Sciences in General
    Type of Medium: Electronic Resource
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  • 2
    ISSN: 1432-0584
    Keywords: Acute parotitis ; l-asparaginase ; Acute leukemia
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary In a patient affected by acute lymphoblastic leukemia (ALL) and subjected to therapy withErwinia l-asparaginase, acute parotitis was observed. Microbiological studies excluded any infectious etiology. Regression of parotitis was spontaneous. This complication has not been previously reported and could be due to the same mechanism of pancreatic injury. The occurrence of acute parotitis needs to be promptly recognized in order to avoid the continuation ofl-asparaginase.
    Type of Medium: Electronic Resource
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  • 3
    ISSN: 1432-0584
    Keywords: Arterial thrombosis ; TIA ; Myocardial infarction ; Congenital protein C deficiency
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary A 49-year-old man was hospitalized for slight paresis of the upper left limb. Thrombosis of the right internal carotid artery was documented by arteriography and digital angiography, which showed evidence of an anastomotic blood flow. He went on anticoagulation treatment. Five years later, after an uneventful period, he was referred to our center for the occurrence of a superficial thrombophlebitis: diagnosis of congenital protein C deficiency was possible in the patient as well as in two of his relatives. Two other subjects with congenital protein C deficiency belonging to two different kindreds, whose illness was diagnosed in our center, suffered from myocardial infarction and TIA, respectively, as the only clinical manifestation; a fourth case, previously described, with recurrent superficial thrombophlebitis, suffered from a TIA when on treatment with stanazolol. These cases indicate that arterial thrombosis or TIA is not an uncommon event in congenital protein C deficiency, even in patients without other risk factors for arterial thrombosis.
    Type of Medium: Electronic Resource
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  • 4
    ISSN: 1432-0584
    Keywords: Extracellular matrix ; Human long-term bone marrow culture
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary The extracellular matrix (ECM) produced by the stromal layer plays a key role in the regulation of commitment and differentiation of hematopoietic cells. Long-term bone marrow culture (LTBMC) allows analysis of the stromal microenvironment. Recently, serum-free LTBMC has been described, but the formation of a classical adherent layer was never observed under these conditions. We have evaluated the effect(s) of a chemically well defined ECM on serum-free and serum-dependent LTBMC. In serum-dependent cultures ECM did not induce a significant increase of hematopoiesis. In serum-free conditions, a marked improvement of hematopoiesis was observed, both in terms of CFU-GM and BFU-E yield and in duration of cultures. A confluent stromal layer was observed only in the presence of ECM. The present results indicate that the addition of ECM to serum-free cultures provides a standardized culture condition, while improving progenitor cell recovery and allowing formation of a confluent stromal layer. Moreover, ECM+ LTBMC may provide a model to study the effect(s) of adhesive proteins and hematopoietic growth factors normally present in serum.
    Type of Medium: Electronic Resource
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  • 5
    Electronic Resource
    Electronic Resource
    Springer
    Digestive diseases and sciences 36 (1991), S. 990-992 
    ISSN: 1573-2568
    Keywords: antithrombin III ; cirrhosis ; hepatocarcinoma
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract It has been reported that hepatoma (HCC) cells produce abnormal proteins such as erytropietin, fibrinogen, prothrombin, and recently, antithrombin III (AT III). In a preliminary report, we reported increased AT III levels in patients bearing HCC independent of their clinical liver status. The present study was performed to assess antithrombin III levels and other serological data present in patients with cirrhosis and in patients with cirrhosis and clinical findings of neoplastic disease. In 70 well-matched patients (47 with cirrhosis and 23 with cirrhosis and proven HCC) serum total cholesterol, albumin, prothrombin, alkaline phosphatase, AFP, aminotransferases, and AT III were determined. Together with AFP and alkaline phosphatase, patients with HCC had higher values of AT III (88±7%) and total cholesterol (184±17 mg/100 ml), as compared with cirrhotic patients (AT III 56±3.6%; total cholesterol 113±5 mg/100 ml) (P〈0.001). No difference was observed between these two groups for albumin, prothrombin, and aminotransferases. In HCC patients, AT III levels were related to the total cholesterol level (R 2=0.317), whereas in the cirrhotic patients it correlated with the prothrombin level (R 2=0.274). These data suggest that in HCC patients a greater rate of synthesis of AT III occurs, whereas in cirrhotic patients lower levels of AT III occur due to impaired synthesis or increased catabolism of the protein. The serial determination of AT III in cirrhotic patients as a means of detecting neoplastic transformation is suggested.
    Type of Medium: Electronic Resource
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  • 6
    Electronic Resource
    Electronic Resource
    Springer
    European journal of plant pathology 96 (1990), S. 65-74 
    ISSN: 1573-8469
    Keywords: air pollution ; foliar injury ; interaction ; predisposition ; weak pathogen
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Description / Table of Contents: Samenvatting De invloed van ozon op de vatbaarheid van boon voorBotrytis cinerea werd onderzocht. Een dag na de blootstelling van vier bonecultivars gedurende 8 uur aan 0, 120, 180 en 260 μg ozon m−3, werden primaire en drietallige bladeren geïnoculeerd met conidiën in water of in een anorganische fosfaatoplossing (Pi). Zichtbare beschadiging door ozon nam met de concentratie toe in alle cultivars. De primaire bladeren waren gevoeliger dan de drietallige. In tegenstelling tot conidiën in water, verooraakten conidiën in de Pi-oplossing lesies op gezonde bladeren. Bij ozonbeschadiging vertoonden bladeren van alle cultivars lesies doorB. cinerea na inoculatie in water. Voor primaire bladeren was het aantal lesies significant gecorreleard met de bladbeschadiging. Na Pi-inoculatie nam bij de ozongevoelige cultivars het aantal lesies ook toe met de concentratie ozon. Echter, de ozontolerante cultivar Groffy vertoonde eerst een afname in de door Pi gestimuleerde infectie bij de laagste ozonconcentratie. De door ozon verhoogde vatbaarheid van drietallige bladeren was minder dan die van primaire bladeren. De resultaten tonen aan dat realistische concentraties van ozon boon vatbaarder maken voorB. cinerea. Deze stijging in vatbaarheid is afhankelijk van het niveau van de ozonbeschadiging die wordt beïnvloed door cultivar, soort blad en ozonconcentratie.
    Notes: Abstract The impact of ozone in predisposingPhaseolus vulgaris toBotrytis cinerea has been investigated. One day after 8 h exposures to 0, 120, 180 and 270 μg ozone m−3, primary and trifoliate leaves of four bean cultivars were detached and inoculated with conidia suspended in water or in an inorganic phosphate (Pi) solution. Visible ozone injury increased with increasing ozone concentrations in all cultivars. Primary leaves were more sensitive than trifoliate leaves. Conidia suspended in Pi solution caused lesions on healthy leaves, whereas conidia in water did not. Ozone-injured leaves of all cultivars showed lesions byB. cinerea after inoculations in water. The number of lesions was significantly correlated with ozone injury for primary leaves. After Pi inoculations, the number of lesions on the ozone-sensitive cultivars also increased with increasing ozone concentrations. However, the ozone-tolerant cultivar Groffy showed first a decrease in the Pistimulated infection at the lowest ozone dosages. The trifoliate leaves of all cultivars were less predisposed to the fungus than the primary leaves. The results indicate that realistic concentrations of ozone enhance the predisposition of bean leaves toB. cinerea. The rate of enhancement depends on the level of ozone-induced injury which was influenced by cultivar, leaf and ozone concentrations.
    Type of Medium: Electronic Resource
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  • 7
    ISSN: 1573-8469
    Keywords: air pollution ; white mould ; grey mould ; pollutant-disease interaction ; predisposition ; necrotrophic pathogens
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract The effects of ozone on the susceptibility of leaves ofPhaseolus vulgaris toSclerotinia sclerotiorum andBotrytis cinerea have been investigated. Seedlings of one ozone-sensitive (‘Pros’) and five relatively ozone-insensitive cultivars (‘Gamin’, ‘Precores’, ‘Groffy’, ‘Narda’, ‘Berna’) were exposed to different ozone concentrations (0, 120, 180 and 270 μg m−3) for 8 h. One day after the exposures, primary leaves were detached and immediately inoculated with spores of either pathogen suspended in water or in a 62.5 mM KH2PO4 (Pi) solution. Visible ozone injury differed between the cultivars and increased with increasing ozone concentration. On the leaves of non-exposed plants, spores of the pathogens suspended in water caused very few lesions, whereas fungal pathogenicity was stimulated by addition of Pi to the inoculum. Ozone-injured leaves of all cultivars exhibited lesions after inoculation of the leaves with the pathogens suspended in water, and the number of lesions was positively correlated with the level of ozone injury for either pathogen and cultivar. The increase in susceptibility of bean leaves in response to increasing ozone concentrations was greater forB. cinerea than forS. sclerotiorum when spores were suspended in water, but was similar when the spores were suspended in Pi. In general, the number of lesions following inoculation with spores in Pi increased with increasing ozone concentration. However, the number of lesions in the ozone-insensitive ‘Groffy’ was reduced by an exposure to 120 μg m−3 but increased with higher concentrations. This pattern of susceptibility response to the pathogens was not found in the other ozone-insensitive cultivars and, thus, did not appear to be related to the inherent ozone-insensitivity in bean.
    Type of Medium: Electronic Resource
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  • 8
    ISSN: 1573-5060
    Keywords: bean ; Botrytis cineres ; inoculation method ; Phaseolus vulgaris ; phosphate ; Sclerotinia sclerotiorum ; screening for resistance
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary A method to inoculate detached bean leaves with Botrytis cinerea or Sclerotinia sclerotiorum without using a carbon-nitrogen source is described. Leaves were incubated in plastic trays that were covered with transparent plastic bags to maintain air humidity above 95%. Spores of either fungus inoculated in water alone were generally unable to cause infection. Additions of KH2PO4 (62.5 mM) or of mixtures of inorganic phosphate and glucose (1.1 or 5.5 mM) to the inoculum stimulated fungal pathogenicity. Also spore concentration (2×105 or 2×↑ 106 spores ml−1) influenced the ability to produce lesions. At 2× ↑106 spores ml−1 both fungi were able to infect primary bean leaves when inoculated in an inorganic phosphate solution. These conditions were used for a preliminary screening for resistance of 15 bean cultivars to both fungi. Only partial resistance to the pathogens was found. Since the procedure is simple and does not require blooming plants, it can be rapidly adopted in screening and breeding programs.
    Type of Medium: Electronic Resource
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