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  • Articles: DFG German National Licenses  (4)
  • 1990-1994  (4)
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  • Articles: DFG German National Licenses  (4)
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Years
Year
  • 1
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    Clinical & experimental allergy 23 (1993), S. 0 
    ISSN: 1365-2222
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: Kikuyu grass (Pennisetum clandeminum) is widely used as a grazing pasture in Africa and, although it is extensively cultivated as a lawn for sports fields and domestic gardens, its allergenicity has never been studied in vitro. Using an extract of Kikuyu grass pollen, polyacrylamide gel electrophoresis. Western blotting and a monoclonal anti-human IgE antibody, the specific IgE binding, in the serum of 160 allergic and non-allergic patients in the Cape Town area, to a Kikuyu grass extract was studied. IgE in the sera of 43/104 known grass-allergic individuals hound to Kikuyu grass on the Western blots. In addition, 4/28 ‘non-allergic’ control subjects were found to have Kikuyu grass-specific IgE. Five different profiles of specific IgE reactivity to Kikuyu grass antigens were oberved. In 29/43 patients, IgE bound to two dominant 48 and 70 kD allergens in the Kikuyu extract. Although a degree of cross-reactivity with Bermuda grass (Cynodon dactylis) was found in immunoabsorption studies, the 48 and 70 kD allergens appear to be unique to Kikuyu grass. Exposure of heparinized blood from Kikuyu grass-positive patients to the Kikuyu extract stimulated the release of histamine from their basophils in vitro. Kikuyu grass pollen is thus identified as an important aero-allergen in South Africa.
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    Clinical & experimental allergy 23 (1993), S. 0 
    ISSN: 1365-2222
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: Using guinea-pig lung membranes and cloned human β2-receptor adrenergic receptors the effects of whole serum, plasma, purified immunoglobulins and cellular activation products on β2-adrenergic receptor ligand binding and function were investigated. Sera from 24 non-asthmatic subjects and 115 asthmatics in different clinical categories were studied. There were no significant differences between antagonist ([125I] cyanopindolol) inhibition mediated by serum, plasma or by purified IgG when the asthmatics were compared with non-asthmatics. There was also no inhibition of 10−6m isoproterenol stimulated cAMP release from L cells expressing human β2-adrenergic receptors, by plasma, DEAE purified IgG fractions from asthmatics and non-asthmatics, or by products of activated platelets or lymphocytes. Since we have no evidence that immunoglobulins from asthmatic subjects exert functional inhibition of human β2-adrenergic receptors we conlcude that autoantibodies to the β2-adrenergic receptors do not play an important functional role in the pathophysiology of asthma.
    Type of Medium: Electronic Resource
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  • 3
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    Clinical & experimental allergy 23 (1993), S. 0 
    ISSN: 1365-2222
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: To investigate a possible genetic basis for reported differences in β-2 receptor expression in atopic subjects, DNA from 42 atopic children (22 asthmatics and 22 with allergic rhinitis) and 30 non-atopic subjects was Southern blotted and Ban-1 restriction fragment polymorphisms (RFLPS) were studied using a 2.6 kb probe of the human β-2 receptor gene. Two alleles 3.1 kb and 2.9 kb were identified. Homozygotes and heterozygotes for the two alleles were found with equal frequency in the atopic patients who had asthma and in those who had allergic rhinitis only. The gene frequencies for the upper and lower alleles were 0.45 and 0.55 respectively. Our studies do not provide evidence for an association between a particular polymorphic form of the human β-2 receptor gene and atopy.
    Type of Medium: Electronic Resource
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  • 4
    Electronic Resource
    Electronic Resource
    Springer
    International journal of earth sciences 83 (1994), S. 212-232 
    ISSN: 1437-3262
    Keywords: South America ; Sands ; Provenance ; Climatic influences
    Source: Springer Online Journal Archives 1860-2000
    Topics: Geosciences
    Notes: Abstract Standard petrographic methods were used to study 604 modern sands from South America, of which 351 came from rivers and 253 from beaches. In spite of the wide geomorphic contrasts, these sands belong to only three great families: (1) an immature Andean family of lithic arenites rich in volcanic and metamorphic grains that covers about 30% of South America; (2) a cratonic association rich in quartz that covers about 62% of the continent; and (3) a transitional, molasse association, which occurs between these two and covers only about 8% of the continent. The grand arithmetic average Q:F: Rf ratio for the entire continent is 60:11:29 and the area-weighted, carbonate-free average Qa:Fa:Rfa is 68:10:22, a superior continental estimate, and the first ever for an entire continent. Rock fragments are the most informative of all the provenance indicators and in South America they range from tropical alterites of the cratonic family through the dominant volcanic and metamorphic grains of the Andean family to biogenic carbonate in beach sands. Carbonate grains are almost totally absent in modern South American rivers because of present day tropical weathering and a long geological evolution that has favored sand production. They are present in many of the beach sands, however, and are most abundant along the tropical Brazilian and Caribbean coasts. Metamorphic grains rival volcanics as earmarks of the Andean family and survive tropical weathering far better than volcanic grains. There is a strong association between continental soil types and the composition of river sand, but the ultimate controls are tectonic history — the ancient landscapes preserved on the Gondwana shields of South America versus the tectonically young landscapes of the Andes — and climate. Climate can either cause ‘leaks’ to a far distant ocean where weathering is retarded by aridity or it can serve as a barrier to continental sand dispersion, where tropical weathering eliminates unstable grains in transit to the sea. When provenance studies are conducted across an entire, unglaciated continent, events in the far distant past need to be considered when seeking fundamental explanations of its contemporary, surficial sands.
    Type of Medium: Electronic Resource
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