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Identification and germline transformation of the ribosomal protein rp21 gene of Drosophila: Complementation analysis with the Minute QIII locus reveals nonidentity (1988)

Kay, Mark A. ; Zhang, J. -Y. ; Jacobs-Lorena, Marcelo

Springer

Molecular genetics and genomics 213 (1988), S. 354-358

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ISSN: 1617-4623

Keywords: Drosophila ; Minute ; Ribosomal protein ; Transformation

Source: Springer Online Journal Archives 1860-2000

Topics: Biology

Notes: Summary Minute loci represent a class of about 50 different Drosophila genes that appear to be functionally related. These genes may code for components of the protein synthetic apparatus. While one Minute locus has been recently shown to code for a ribosomal protein, it is not yet known whether any of the other Minute loci also code for ribosomal proteins. We have addressed this question by a combined molecular and genetic approach. In this report, a cloned DNA encoding the ribosomal protein rp21 is partially characterized. The rp21 gene maps to the same region (region 80 of chromosome 3L) as the temperature-sensitive Minute QIII gene. Using P-element mediated transformation, the rp21 gene was transformed into the germline of Drosophila. RNA blot experiments revealed that the transformed gene is expressed in transgenic flies. However, genetic complementation analysis indicated that the QIII locus and the rp21 gene are not identical. Implications of these findings for the relationship between Minutes and ribosomal protein genes are discussed.

Type of Medium: Electronic Resource

URL: http://dx.doi.org/10.1007/BF00339602

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Optical activity based on macromolecular asymmetryPart XXVI of Haloaldehyde Polymers; Part XXV : E.G. Brame, A. M. Raevsky, G. K. Semin, G. D. Jaycox, and O. Vogl, Polymer Bulletin 10(11/12), 521 (1983) (1985)

Vogl, Otto ; Corley, L. Steven ; Harris, William J. ; [et al.]

New York : Wiley-Blackwell

Die Makromolekulare Chemie 13 (1985), S. 1-12

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ISSN: 0025-116X

Keywords: Chemistry ; Polymer and Materials Science

Source: Wiley InterScience Backfile Collection 1832-2000

Topics: Chemistry and Pharmacology , Physics

Notes: Optically active isotactic polychloral was prepared in which the optical activity comes exclusively from molecular asymmetry (i.e., helical conformation). Molecular asymmetry requires a high conformational energy barrier for the polymer backbone, and an asymmetric initiator to induce a predominance of one helical screw-sense. Polychloral meets the criteria. Asymmetric initiators used to obtain optically active polychloral include tetramethyl ammonium (+)- or (-)-0-acetylmandelate, tetramethylammonium (+)- or (-)-0-methylmandelate, the lithiumalkoxides of methyl (+)- or (-)-mandelate, lithium cholesten-3β-oxide, lithium cholestan-3β-oxide, and lithium (+)- or (-)-2-octanoxide. Using the above initiators at 0.5 mol %, a maximum specific rotation of [α] D25 = 5000 was obtained for polychloral. Errors in specific rotation were typically ±7%.