ISSN:
1617-4623
Keywords:
Erwinia
;
λ::Tn5 mutagenesis
;
Mutants of reduced virulence
;
Pectolytic enzyme mutants
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Summary The blackleg pathogen Erwinia carotovora subsp. atroseptica (Eca) causes an economically important disease of potatoes. We selected a genetically amenable Eca strain for the genetic analysis of virulence. Tn5 mutagenesis was used to generate nine mutants which exhibited reduced virulence (Rvi-) of strain SCRI1043. Following physiological characterisation, mutants were divided into three classes: (1) auxotrophs; (2) extracellular enzyme mutants; and (3) a growth rate mutant. The isolation of these Rvi- mutants has allowed us to consider some factors that affect Eca virulence.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00330953
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