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  • Articles: DFG German National Licenses  (2)
  • 1980-1984  (1)
  • 1970-1974  (1)
  • 1965-1969
  • Colorimetric iron determination  (1)
  • Hemoglobin content in blood, colorimetric iron determination  (1)
  • Proximal Kidney Tubule  (1)
Source
  • Articles: DFG German National Licenses  (2)
Material
Years
  • 1980-1984  (1)
  • 1970-1974  (1)
  • 1965-1969
Year
  • 1
    ISSN: 1437-1596
    Keywords: Colorimetric iron determination ; Hemoglobin content in blood, colorimetric iron determination ; Eisenbestimmung, kolorimetrisch ; Hämoglobingehalt im Blut, kolorimetrische Eisenbestimmung
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine , Law
    Description / Table of Contents: Zusammenfassung Es wird eine einfache und genaue Methode zur Bestimmung von Eisen im Blut unter Verwendung von Säureaufschluß im Heizblock beschrieben. Der Aufschluß benötigt wenig Mühe und erlaubt die Verarbeitung einer großen Zahl von Proben. Der verdünnte Aufschluß wird zur kolorimetrischen Eisenbestimmung mittels o-Phenanthrolin benutzt und die entsprechende Hämoglobinkonzentration im Blut berechnet. Es zeigte sich eine gute Korrelation der Ergebnisse mit der beschriebenen und der Cyanmethämoglobin-Methode anhand von 21 Standardproben, die 4,9 bis 26,8 g/dl Hämoglobin enthielten.
    Notes: Summary A simple and accurate determination of iron in blood involving acid digestion in a heating block is described. The digestion requires little attention and permits the handling of large numbers of samples. The diluted digest is used for the colorimetric determination of iron using o-phenanthroline, and the equivalent hemoglobin in blood is calculated. There was a good correlation between the results of the present method and the cyanmethemoglobin method with 21 standard blood samples containing 4.9–26.8 g/dl of hemoglobin.
    Type of Medium: Electronic Resource
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  • 2
    ISSN: 1432-2013
    Keywords: Proximal Kidney Tubule ; Mercurials ; SH Reagents ; Site Group Reagents ; Transtubular Transport
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary The effects of site group reagents were tested on the following transport processes of the proximal convolution. Isotonic Na+ absorption, evaluated by the shrinking droplet procedure, histidine and glucose transport, evaluated by measuring the respective transtubular concentration difference at zero substance and water net flux. The test substances were applied either by continuous microperfusion of the peritubular capillaries or by luminal perfusion prior to the transport tests or by addition to the luminal test solution. The SH reagents (0.2 mM) N-ethylmaleimide,p-chloromercuribenzoate (pCMB) 3,6-bis-(acetatomercurimethyl)dioxane and Mersalyl (Salyrgane) caused 50% inhibition of the isotonic Na+ absorption in approximately 1.5 min when applied to the capillary perfusate. The same effect was reached in 2–3 min by 0.2 mMp-chloromercuriphenylsulfonate, benzamido-4-iodo-acetylstilbene-2,5-disulfonate and 2,2′-dihydroperoxy-2,2′-dibutylperoxide. However, the large molecular SH reagentspCMB-dextran T10 and benzoxanthene-3,4-dicarboxylic-N-iodoacetyloligoprolyl-2-aminoethylimid, did not inhibit the isotonic Na+ absorption. If an inhibitory effect was observed on the Na+ transport its onset was faster, when the substance was applied from the blood site than when it was given from the tubular lumen. Because SH reagents inhibit the isotonic Na transport faster when applied from the blood side, and because SH reagents with MW up to 690 are inhibitory whereas larger ones with MW over 1700 are not, it seems that they exert their inhibitory action on SH groups located a) predominantly on the blood side and b) deep within the membrane and not at the surface. Histidine- and glucose transport was inhibited only when the sodium transport was inhibited considerably. The oxygen consumption of teased kidney slices is not inhibited by 0.2 mMpCMB or Mersalyl within 10 min, but it is inhibited considerably by 1 mM of these substances in the same period of incubation time. The COOH reagents N,N′-carbonyl-diimidazole and N-ethyl-N′-(3-dimethyl-aminopropyl)carbodiimid (10 mM) and the NH2 reagents 4-acetamido-4′-isothiocyanatostilbene-2,2′-disulfonic acid, 2 Na+ (SITS) (1 mM) as well as danslychloride (applied from the lumen at 5 mM in paraffin oil) did not inhibit the isotonic Na+ absorption.
    Type of Medium: Electronic Resource
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