ISSN:
1365-2133
Source:
Blackwell Publishing Journal Backfiles 1879-2005
Topics:
Medicine
Notes:
Fresh, not chemically fixed, glycerol-cryoprotected material from skin lesions of histiocytosis-X (Letterer-Siwe disease) was examined by the freeze-fracture technique. Fracture appearances of the cytoplasmic X-granules characteristic of cells of histiocytosis-X were established, and the accuracy of previous three-dimensional reconstructions of variations, in overall shape of granules based upon stained thin sections was confirmed. These variations, therefore, are not artifacts of conventional tissue processing. Little or nothing of the internal structure of X-granules is seen in replicas, and the technique provides no new information concerning their origin or mode of formation.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1111/j.1365-2133.1973.tb07582.x
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