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  • Articles: DFG German National Licenses  (3)
  • 1980-1984  (3)
  • Taxol  (3)
  • 1
    Electronic Resource
    Electronic Resource
    Indirect immunofluorescent staining of the microtubules in interphase and mitotic amoebae of the myxomycetePhysarum polycephalum (1984)
    Springer
    Protoplasma 120 (1984), S. 43-50 
    Details
    ISSN: 1615-6102
    Keywords: Aster ; Immunofluorescence ; Mitosis ; Myxomycetes ; Physarum ; Taxol ; Tubulin
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The microtubules ofPhysarum amoebae have been decorated with rat antibodies against yeast tubulin. The indirect fluorescent staining observed in interphase amoebae and in flagellated amoebae is consistent with the three-dimensional reconstructions previously deduced from electron microscopic studies. Mitotic amoebae exhibit a pattern of fluorescence which is similar to that exhibited by mammalian cells and is consistent with the previous electron microscopic studies, except that we also observe pole-pole microtubule fibers during metaphase and anaphase and the presence of a typical midbody during cytokinesis. The various types of tripolar mitosis which are observed suggest that there is a regulatory mechanism allowing the formation of pseudo-bipolar mitotic apparatuses in amoebae possessing more than two mitotic centers during mitosis. The mitotic center, located in the middle of the centrosphere, is not fluorescent after staining of the monoasters induced with taxol suggesting the absence of tubulin in the mitotic center.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01287616
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Stabilization of monoasters by taxol in the amoebae ofPhysarum polycephalum (Myxomycetes) (1982)
    Springer
    Protoplasma 113 (1982), S. 44-56 
    Details
    ISSN: 1615-6102
    Keywords: Taxol ; Amoebae ; Physarum ; Myxomycetes ; Centrosphere ; Mitosis ; Microtubule ; Centriole
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Although the plasmodial stage of the MyxomycetePhysarum polycephalum was unaffected with 200 μM taxol, the amoebal stage was sensitive to 10 μM taxol. The first effect of taxol resulted in an accumulation of cells blocked as a monopolar centrosphere surrounded by condensed chromosomes. In 79% of cases these monoasters contained two pairs of centrioles. The mitotic block in a monopolar stage in the presence of taxol delayed the occurrence of late mitotic events such as chromosome decondensation and formation of the nuclear envelope. Escape from the monopolar centrosphere stage and formation of multinucleated amoebae involved a transient monopolar reconstruction stage in which a long microtubular bundle interacted with a small chromosomal mass outside the monoaster.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01283038
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    Paper (German National Licenses)
    Fulltext
  • 3
    Electronic Resource
    Electronic Resource
    Spatial relationships between centrioles and the centrosphere in monoasters induced by taxol inPhysarum polycephalum amoebae (1982)
    Springer
    Protoplasma 113 (1982), S. 69-79 
    Details
    ISSN: 1615-6102
    Keywords: Taxol ; Amoebae ; Physarum ; Myxomycetes ; Centrosphere ; Mitosis ; Centriole
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Monoasters induced by taxol in the amoebae of the MyxomycetePhysarum polycephalum show an unusual tridimensional location of the centrioles. Tridimensional reconstructions of individual monoasters with either two or four centrioles show that the position of centrioles is not random. The characteristics of these monoasters suggest that the centrioles are not linked to the mitotic center in the monoaster since mitotic centers completely devoid of centrioles in adjacent or central location are observed. However, the preferential centrifugal orientation of the centrioles in the centrosphere induced by taxol suggests that centrioles are initially located in the mitotic center in agreement with the attachment of the centrioles to the mitotic center during interphase.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01283040
    Library Location Call Number Volume/Issue/Year Availability
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    Paper (German National Licenses)
    Fulltext
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