ISSN:
1573-5044
Keywords:
carrot
;
somatic embryo
;
protoplast
;
cell density
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract Protoplasts were isolated enzymatically from synchronously induced globular somatic embryos from a carrot suspension culture. Among the macerating enzymes tested, Driselase was the most effective for release of protoplasts from embryos. A higher medium osmolarity was required for the isolation of protoplasts from embryos than from undifferentiated cells. Protoplasts from embryos were smaller than protoplasts from undifferentiated cells. On step gradients of Ficoll, protoplasts from embryos gave one major band. Protoplasts from undifferentiated cells gave two major bands, one lighter and the other heavier than the protoplasts from embryos.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF02318917
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