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  • Articles: DFG German National Licenses  (8)
  • 1975-1979  (3)
  • 1965-1969  (5)
  • Life and Medical Sciences  (8)
  • 1
    Electronic Resource
    Electronic Resource
    The sudanophilic lipid of normal and regenerating limb tissues of the adult newt, Diemictylus viridescensThis investigation was supported by a Public Health Service research grant GM06208 from the National Institute of General Medical Sciences. (1966)
    New York, NY : Wiley-Blackwell
    Journal of Morphology 118 (1966), S. 57-77 
    Details
    ISSN: 0362-2525
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: The most intense and widely distributed sudanophilic responses of cryostat-sectioned newt limb tissues were obtained with a simultaneous fix and stain procedure of 1:1 10% formal-calcium and sudan black B. Droplets and globules of lipid mixtures and rodlets (mitochondria) were typical responses distributed within the epidermis, subcutaneous glands, dermis and other connective tissues, striated muscle (also with positive fibrils), tunics of blood vessels, and blood cells. A prominent droplet response was located subjacent to the adepidermal basement membrane. The myelin of brachial nerve stained intensely.In regenerating limbs, the wound epithelium response was comparable to that of epidermis. Post-amputational lipophanerosis of injured muscle and brachial nerves was observed. The retrograde degeneration of nerve myelin was extensive, and continued into the early differentiative phase of regeneration. Lipid-engorged macrophages were prominent among the injured tissues, distal to these, and within the wound epithelium.The regeneration blastema revealed a large quantity of sudanophilic lipid. Prominent droplet and rodlet responses were typical of the myelinating regenerating nerves. The response of regenerating muscle equaled that of the mature stump fibers. The cells of the regenerating chondroskeleton contained sudanophilic lipid.Organic solvents such as acetone, ether, chloroform and chloroform:methanol reduced or prevented the sudanophilic responses. Sudan red 7B revealed less lipid than did sudan black B. A fixation effect was demonstrated with post-chromated formalcalcium, and chromic-formalin fixed sections. In the latter preparations, swollen-bodies, identified as mitochondria, stained intensely.
    Additional Material: 10 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jmor.1051180105
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  • 2
    Electronic Resource
    Electronic Resource
    Lipoprotein in the normal and regenerating limb tissues of the adult newt, Diemictylus viridescensThis investigation was supported by Public Health Service Research grant GM06208 from the National Institute of General Medical Sciences. (1966)
    New York, NY [u.a.] : Wiley-Blackwell
    The @Anatomical Record 155 (1966), S. 65-75 
    Details
    ISSN: 0003-276X
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Medicine
    Notes: Lipids tightly bound to cell and tissue proteins (lipoproteins) were investigated with the acetone-sudan procedure in cryostat-cut sections of normal and regenerating limb tissues of the adult newt, Diemictylus viridescens.Nuclear and cytolplasmic membranes of all tissues stained moderately black; the nucleo- and cytoplasm stained less intensely than their membranes. Connective tissue fibers of the dermis, mysial and neural sheaths, and tunics of blood vessels yielded intense responses. In striated muscle of the limb, myofibrils and associated striations responded strongly. Nerve myelin responded weakly.In the preblastemic regenerate, the fibrous adepidermal basement membrane terminated abruptly at the surface of amputation. A distal fibrocellular residue was evident in continuity with the retrograde degeneration of amputation-injured muscle: observations suggest a possible contribution to the fibrocellular reticulum from myofibrils. The regeneration blastema appears isolated from proximal limb tissues by the intervening fibrocellular reticulum. The response of the blastema cell is relatively weaker than that of other cells and tissues. Regenerating muscle is recognized by the appearance of prominently stained myofibrils in myoblastic extensions off the limb stump musculature. The matrix and chondrocytes of regenerating cartilage stain for lipoprotein, with the osteoid of osteogenic centers responding strongly.Polychrome responses were obtained from hyalin-bodies within interphase nuclei, and from chromosomes in mitosis, suggesting that chromosomal lipid has been stained.Supporting the lipidic character of the observed responses is the negative reaction following long term lipid extraction in warm chloroform: methanol.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/ar.1091550108
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  • 3
    Electronic Resource
    Electronic Resource
    The fine structure of limb tissues of the adult newt, Diemictylus viridescensThis investigation was supported by a research grant GM 06208, and a graduate training grant 8T1-HD-16, from the Public Health Service. (1967)
    New York, NY : Wiley-Blackwell
    Journal of Morphology 123 (1967), S. 251-269 
    Details
    ISSN: 0362-2525
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: The limb tissues of the adult newt investigated for their fine structure include epidermis, subcutaneous glands, dermis, striated muscle, peripheral nerves and blood vessels. This survey complements and extends previous observations, emphasizing intercellular junctions, and the ubiquitous “glycocalyx” (= polysaccharide-protein lamella, around cells and adjacent to epithelia).Our survey touches on the characteristic tonofilaments, intercellular desmosomes and basal hemidesmosomes of the epidermis. The subcutaneous glands consist of secretory cells with a granular product, and myoepithelial cells; intercellular desmosomes are present. The adepidermal reticulum of collagen fibrils reveals periodic regions of intersecting fibrils ( = nodules), and fibril continuity with the underlying dermis: a striking feature is the adipose tissue closely applied to the adepidermal reticulum. The limb striated muscle displays typical banded myofibrils, and a triad system with centrotubules in the I-band close to the Z-band: terminal sacs of sarcoplasmic reticulum complete the triad system. A particularly prominent glycocalyx is applied to the surface of the sarcolemma. The peripheral nerves of the limb possess connective tissue sheaths with prominent vesiculation of the cell membranes, and an occasional intercellular desmosomal junction. Blood vessels typically have endothelial cells with prominently vesiculated plasma membranes.This investigation serves as the basis for recognizing the fine structure of tissue responses to trauma, their repair, and regeneration.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jmor.1051230305
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  • 4
    Electronic Resource
    Electronic Resource
    Lipid of normal and regenerating limb tissues of the adult newt, Diemictylus viridescens: Acidic and non-acidic lipids, phospholipids, and cholesterolThis investigation was supported by a Public Health Service Research grant GM06208, from the National Institute of General Medical Sciences. (1966)
    New York, NY : Wiley-Blackwell
    Journal of Morphology 118 (1966), S. 353-365 
    Details
    ISSN: 0362-2525
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: Cryostat-cut sections of unamputated and amputated-regenerating limbs of the adult newt were examined following the Nile blue test for acidic and non-acidic lipids, the acid hematein and plasmal tests for phospholipids, and a Schultz test for cholesterol.Triglycerides (Nile blue test) are prominent in dermis and macrophages: triglyceride droplets are scattered in epidermis, wound epithelium, and regeneration blastema. Fatty acids (Nile blue test) are present in all tissues of the normal and regenerating limb: nerve myelin contains relatively little free fatty acid, while macrophages appear to contain the least amount of this lipidic substance.Plasmalogens (plasmal test) are prominent in the myelin of nerves, and macrophages: a weak cytoplasmic reaction obtains in the epidermis, subcutaneous glands, striated muscle, tunics of blood vessels, wound epithelium, blastema cells, chondrocytes, perichondrium and periosteum.Mitochondria responding for cephalin, lecithin, and sphingomyelin (acid hematein test) are ubiquitously distributed among the cells and tissues of the normal and regenerating limb. These phosphatides are prominent in nerve myelin, macrophages, and in dermal droplets: a variable response obtains from the myofibrils of striated muscle.Cholesterol (Schultz test) was demonstrated only in nerve myelin and in macrophages associated with injured nerves.Particular attention was paid to the lipid responses of the regeneration blastema, and the conclusion was reached that not all of the lipid previously demonstrated with sudan dyes was characterized by the current series of lipid tests.A modified Nile blue sulfate test that promises greater specificity in distinguishing between acidic and non-acidic lipids is introduced.
    Additional Material: 7 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jmor.1051180305
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  • 5
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    Electronic Resource
    The fine structure of tissues in the amputated-regenerating limb of the adult newt, Diemictylus viridescensThis investigation was supported by a research grant GM 06208, and a graduate training grant 8T1-HD-16, from the Public Health Service. (1967)
    New York, NY : Wiley-Blackwell
    Journal of Morphology 123 (1967), S. 271-311 
    Details
    ISSN: 0362-2525
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: We have examined the fine structure of injured, repairing and regenerating tissues of the amputated forelimbs of the adult newt: observations were made on wound epithelium, nerves, striated muscle, the regeneration blastema, connective tissues and cartilage.The wound epithelium shows little modulation from the adjacent epidermis: a subjacent glycocalyx and hemidesmosomes are initially absent. Some cells reveal phagocytic activity. As regeneration progresses, a glycocalyx, hemidesmosomes, and an adepidermal reticulum of fibers develop.Nerve fiber degeneration and regeneration lie side by side in the same nerve bundle. Myelin degeneration is evident within 24 hours; neural connective tissues loosen and fibers appear to spread apart.Striated muscle fibers display a nonuniform response to trauma, due to unequal degree of injury inflicted by amputation. The moderately and greatly injured fibers lose their distal fibril organization. There is a numerical increase in mitochondria, and occasionally a pyknotic nucleus is present. The myofiber glycocalyx appears to resist destruction, even when cell degeneration is extensive. Satellite cells have been observed intimately in contact with injured myofibers. Myogenesis is accompanied by an increase in collagen fibrillogenesis associated with adjacent fibroblasts. Many of these latter cells appear to contain intracellular fibrils: the significance of these observations are discussed, and a basis for intracellular fibrillogenesis and tropocollagen accretion is proposed.The fine structure of the regeneration blastema cell agrees with the description offered by others for the adult newt limb regeneration.Early prochondral condensation of blastema cells is described briefly.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jmor.1051230306
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  • 6
    Electronic Resource
    Electronic Resource
    The effect of large unilateral cortical lesions on rubrospinal tract sprouting in newborn rats (1977)
    New York, NY [u.a.] : Wiley-Blackwell
    American Journal of Anatomy 149 (1977), S. 39-45 
    Details
    ISSN: 0002-9106
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Medicine
    Notes: Many previous reports have demonstrated the development of aberrant neural connections in response to neonatal brain lesions. This investigation was undertaken to study possible alterations, particularly axonal sprouting, in rodent rubrospinal projections after neonatal destruction of the corticospinal tract through frontal cortical ablation. The neonatal ablations were made by aspiration in 1 to 2-day-old rats under hypothermic anesthesia. At three to six months after neonatal surgery, the rubrospinal tracts were ablated bilaterally in these same animals as well as in controls, by stereotaxically transecting the ventral tegmental decussation. Animals were killed two to six days after adult surgery, and rubrospinal projections were demonstrated using the Fink-Heimer degeneration stain. No differences in the pattern of rubrospinal projections were observed between animals with neonatal cortical lesions and controls. In all animals rubrospinal projections were located primarily in Rexed's lamina VI with a slight distribution into lamina V and the dorsal portion of lamina VII. Various hypotheses explaining the lack of rubrospinal sprouting after neonatal cortical lesions are presented, along with possible experiments to test these hypotheses.
    Additional Material: 3 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/aja.1001490104
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  • 7
    Electronic Resource
    Electronic Resource
    Retrograde changes in Clarke's column following neonatal hemicerebellectomy in the rat (1979)
    New York, NY [u.a.] : Wiley-Blackwell
    American Journal of Anatomy 156 (1979), S. 533-542 
    Details
    ISSN: 0002-9106
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Medicine
    Notes: Long-Evans black-hooded rat pups underwent hemicerebellectomies at various postnatal ages. The animals were killed at 28 days of age and thoracolumbar segments of the spinal cord were removed and processed according to Golgi and Nissl techniques. Hemicerebellectomy at two and three days postnatally produced, in addition to cellular hypotrophy in Clarke's column, dendritic alterations which included a decrease in the number of primary dendrites and a reduction of the remaining dendrites to truncated stubs. Hemicerebellectomy at 21 days postnatally had little effect on either cell size or dendritic arborization. The role of the target nucleus in maintaining cell growth and dendritic arborization during critical developmental periods is discussed.
    Additional Material: 6 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/aja.1001560407
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  • 8
    Electronic Resource
    Electronic Resource
    Separation of megakaryocytes from rat bone marrow cells using velocity sedimentation in an isokinetic gradient of ficoll in tissue culture mediumThis investigation was supported by Public Health Service Grants CA-15089 from the National Cancer Institute, CA-16764 from the National Cancer Institute through the National Large Bowel Cancer Project, CA-16430 from the National Cancer Institute through the National Prostatic Cancer Project. DE-2670 from the National Institute of Dental Research and by American Cancer Society Grant PDT-9B. Dr. Pretlow is supported by NIH Research Career Development Award K4-CA-70584-03. (1976)
    New York, NY [u.a.] : Wiley-Blackwell
    Journal of Cellular Physiology 88 (1976), S. 317-322 
    Details
    ISSN: 0021-9541
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: A technique for the purification of rat megakaryocytes is described. Velocity sedimentation in a previously described isokinetic gradient of Ficoll (polysucrose) in tissue culture medium was more effective than isopycnic sedimentation for the purification of megakaryocytes and resulted in preparations of megakaryocytes which contained 2.4 ± 0.8% (range 1.85-3.60%) megakaryocytes. Megakaryocytes exhibited a broad range of density between 1.06 and 1.15 gm/ml. The inaccuracy which is inherent in the use of velocity sedimentation without isopycnic sedimentation as a means of particle size analysis is discussed.
    Additional Material: 2 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jcp.1040880307
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