ISSN:
1432-1912
Keywords:
Phenylbutazone
;
Cell Culture
;
Serum-Protein Binding
Source:
Springer Online Journal Archives 1860-2000
Topics:
Medicine
Notes:
Summary The influence of phenylbutazone (Butazolidin®) on the growth of mouse fibroblasts (L 929) in-vitro was studied. The substance was added in concentrations of 190, 364 and 667 μg/ml to cultures in media containing different amounts of serum ranging from 10 to 50%. The inhibition of cell growth was found to be proportional to the concentration of phenylbutazone. Furthermore, the inhibition of growth was found to be inversely proportional to the serum content of the media. The free and protein bound 14C-phenylbutazone present in Eagle's basal medium, supplemented with different volumes of foetal bovine serum, was determined by both equilibrium dialysis and ultracentrifugation method. The results indicated that the unbound phenylbutazone was responsible for the inhibition of growth. In the culture medium containing added serum protein the percentage of bound phenylbutazone was found to be relatively small, in contrast to in-vivo where, at therapeutic levels of the drug in plasma, up to about 98% is bound.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00501421
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