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  • Articles: DFG German National Licenses  (1)
  • 1970-1974  (1)
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  • Articles: DFG German National Licenses  (1)
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    Electronic Resource
    Electronic Resource
    Large scale production of bacteriophage Φ × 174 (1973)
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 15 (1973), S. 927-932 
    Details
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Bacteriophage Φ × 174 was produced in 20 and 200 liter fermentors. The phage was concentrated without any loss of infectivity by precipitation with 10% (w/v) Poly(ethylene glycol) 6000 in 0.5M NaCl. The total yield of a 100 liter culture is around 1016 infectious particles. The final purification step in a CsCl gradient results in a 60 to 80% inactivation.
    Additional Material: 1 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/bit.260150510
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