ISSN:
1432-2307
Source:
Springer Online Journal Archives 1860-2000
Topics:
Medicine
Notes:
Summary The auricular lymph nodes of SPF guinea pigs were examined light microscopically at various stages (30 min to 14 days) after subcutaneous injection of Listeria monocytogenes. The bacteria entered the lymph node parenchyma through the marginal sinuses and were phagocytosed by polymorphonuclear leukocytes and monocytes at early stages of the infection (30–60 min). Whereas retothelial cells of the sinuses did not phagocytose bacteria, submarginal reticulum cells showed a high bacterium-phagocytosing activity. Starting at 12 hours after inoculation lymphocyte activation occurred in the interfollicular diffuse lymphatic tissue of the cortex and in the paracortex. The activated lymphocytes were released into the sinuses. There was also paracortical distension, which reached its peak on the 6th day of infection. The submarginal area at the junction of the afferent lymphatic with the cortex and the perivascular regions of the lymph node paracortex were the preferred sites of granuloma formation. On the 8th day with a dose of 106 bacteria the macrophages of the granulomas revealed only unidentifiable debris in the cytoplasm, whereas in the earlier lesions a large number of mononuclear phagocytes, especially in the submarginal granulomas, contained many intracytoplasmic listeria. The granulomas gradually became smaller and smaller. With larger infectual doses the process did not subside within the experimental period; the granulomas developed the appearance of “reticulocytäre abszedierende Lymphadenitis”.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF01201316
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