ISSN:
1432-0886
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
,
Medicine
Notes:
Summary A cytological investigation of honeybee tissues was pursued by two techniques. The first was an estimation of nuclear volume in large samples of nuclei; the second was a determination of DNA per nucleus by absorption photometry of Feulgen-stained nuclei. It was found that nuclear volume frequency peaks were as follows: InMalphigian tubule cells—drone 185 cubic micra, worker 135, queen 135 and265, and worker pupa 25 cubic micra. Insmall intestine epithelium cells—drone 285, worker235 and 475, queen105 and 235, and worker pupa 85 cubic micra. (The major class in each case is underlined.) The results of DNA determinations gave evidence for the following: a) High degrees of polysomaty occur in honeybee tissues. b) A single tissue may have more than one characteristic degree of polysomaty. c) A rough, positive correlation exists between degree of ploidy and secretory activity. d) Male tissues have about the same chromosome numbers as comparable female tissues. e) Both male and female tissues contain haploid nuclei. The concentration of DNA in pupal testis is less than that of any other tissue studied while the concentration of DNA in pupa small intestine is greater than that in the adult organ. In two tissues at least, the concentration of DNA in male nuclei is less than that in the worker. It was concluded that there is not always a relationship between nuclear volume and DNA content in honeybee tissues.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF01259952
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