ISSN:
1573-5060
Keywords:
chicory
;
Cichorium intybus
;
protoplast fusion
;
tetraploids
Source:
Springer Online Journal Archives 1860-2000
Topics:
Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
Notes:
Summary Fusions of chicory leaf protoplasts were realized in order to obtain a higher number of tetraploid plants compared to the traditional doubling techniques with colchicine. Thanks to the fusion technique, using a mixture of PEG, DMSO and a solution of CaCl2 (pH 10.5), 25% tetraploid (2n=36) plants were obtained. Among the 167 regenerated plants, only one aneuploid of a near tetraploid level (2n=33) was identified.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00036089
Permalink