ISSN:
1573-5001
Keywords:
3D HCCH-TOCSY
;
Solvent suppression
;
Pulsed-field gradients
;
Coherence pathway selection
;
Protein side-chain assignments
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
,
Chemistry and Pharmacology
Notes:
Summary A pulsed-field gradient-enhanced, heteronuclear cross-polarization-driven, 3D HCCH-TOCSY experiment is described, which in a single scan can achieve nearly ideal solvent suppression for protein samples in H2O solution. The 3D experiment can be transformed without additional pre- or post-processing, thus leaving solute resonances at the solvent resonance position undisturbed and easily identifiable. As the gradients are used in combination with a 13C z-filter, only minimal relaxation losses are encountered as compared to non-gradient versions.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00208812
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