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  • Articles: DFG German National Licenses  (2)
  • 6b gene  (1)
  • fringe patterns  (1)
  • 1
    Electronic Resource
    Electronic Resource
    The Agrobacterium tumefaciens C58-6b gene confers resistance to N6-benzyladenine without modifying cytokinin metabolism in tobacco seedlings (1999)
    Springer
    Planta 209 (1999), S. 453-461 
    Details
    ISSN: 1432-2048
    Keywords: Key words:Agrobacterium (6b gene) ; 6b gene ; Cytokinin resistance ; Nicotiana (transformed) ; Transgenic plant (tobacco)
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract. The 6b gene of Agrobacterium tumefaciens has been demonstrated to modify the activity of the plant growth regulators, auxin and cytokinin. To study the possible mode of action of the gene, tobacco (Nicotiana tabacum L. cv. Samsun) plants were transformed with the A. tumefaciens C58-6b gene. Seeds obtained from morphologically normal transgenic as well as wild-type plants were germinated on media supplemented with growth-inhibitory levels of cytokinin, N6-benzyladenine (BA). The transgenic seedlings showed increased resistance to cytokinins, as reflected by continuous shoot development, whereas further growth of the wild-type plants beyond the cotyledonary stage was inhibited. Concurrently, the levels of 6b gene transcripts in transgenic seedlings increased greatly upon BA treatment. Since glucosylation of BA represents the main inactivation mechanism of the hormone, we analyzed BA glucoside formation during the early stages of seedling growth. Intracellular levels of the major BA metabolite, N6-benzyladenine-7-glucoside (80–92%), as well as other BA-derived components were found to be comparable in transgenic and wild-type seedlings. Therefore, increased resistance of the C58-6b transgenic seedlings to cytokinins could not be directly attributed to enhanced BA glucosylation and subsequent hormone inactivation.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s004250050748
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Image analysis of oil film interferometry — a method of measuring wall shear stress distributions (1992)
    Springer
    Flow, turbulence and combustion 49 (1992), S. 261-270 
    Details
    ISSN: 1573-1987
    Keywords: interferometry ; shear stress measurement ; image processing ; fringe patterns
    Source: Springer Online Journal Archives 1860-2000
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
    Notes: Abstract A method is described of measuring a wall shear stress distribution that varies in the direction of the flow. Variations in the height of a very thin oil film moving under the boundary layer generate interference fringes, which are recorded and digitised using image processing equipment. The evolution of the film surface in space and time can be reconstructed from the interference fringe patterns and used to calculate the shear stress field. This reconstruction is achieved by comparing the picture data with images that were calculated for prescribed heights that are adjusted iteratively, until the calculated intensities match the data. The method is applied to a flow approaching a step, and the results are compared with pulsed-wire measurements.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00384626
    Library Location Call Number Volume/Issue/Year Availability
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    Paper (German National Licenses)
    Fulltext
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