ISSN:
1432-1912
Keywords:
DOPA-Pool
;
DOPA Decarboxylase
;
Adrenal Gland
;
Catecholamines
;
DOPA-pool
;
DOPA Decarboxylase
;
Nebenniere
;
Katecholamine
Source:
Springer Online Journal Archives 1860-2000
Topics:
Medicine
Notes:
Summary 3H-tyrosine was administered to cats as a continuous i.v. infusion of 30 min duration after inhibition of DOPA-decarboxylase by N-methyl-N′(3-hydroxybenzyl) hydrazine dihydrogen phosphate (= NSD-1034). Subsequently, the content of3H-DOPA in the arterial blood, adrenal glands and adrenal venous blood was measured. For this purpose DOPA was separated from tyrosine and other radioactive tyrosine catabolites by ion exchange chromatography after adsorption onto and elution from alumina. 3H-DOPA was not only detectable in the adrenal glands and in the adrenal venous blood but also in the arterial blood. The content of DOPA in the arterial blood increased linearly during the 30 min infusion period and was 0.5 mμmole/ml at the end of the experiment. This corresponds to a DOPA content in the total blood volume of about 27.5 mμmole/kg body weight. Both adrenals synthesized 0.3 mμmole DOPA per minute and per kg body weight. The significance of the formation of a DOPA pool for the therapeutic use of DOPA decarboxylase inhibitors is discussed.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00997782
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