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  • Articles: DFG German National Licenses  (2)
  • Aldolase  (1)
  • Bone density measurement  (1)
  • 1
    ISSN: 1432-0827
    Keywords: Bone density measurement ; Dual energy X-ray absorptiometry ; Collagen type I ; Biochemical bone markers ; b-quotient
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine , Physics
    Notes: Abstract In contrast to medical imaging, the biochemical markers allow a more frequent determination and are not as invasive as histomorphometric methods. We investigated biochemical markers of type I collagen compared with bone density measurements in 85 females between 41 and 89 years of age (median: 57 years). The bone density measurements were performed by dual-energy X-ray absorptiometry (DXA) on the lumbar spine (L1–4). The bone density measurements were stated as percentage of the norm. All patients were divided into three groups: I=〈80%; II=80–120%; III=〉120%. Based on this classification the median concentration of the I-carboxyterminal propeptide of type I procollagen in serum (S-PICP) as an anabolic marker of type I collagen increased significantly with rising bone density: I 65.0* μg/liter (interquartile range: 52.1–78.0 μg/liter); II 85.9* μg/liter (52.1–115.5 μg/liter); III 81.4 μg/liter (62.0–101.0 μg/liter); * P〈0.05. The concentration of urinary pyridinolines (U-PYR) as a marker for degradation of type I collagen decreased. The I-carboxyterminal telopeptide (S-ICTP) and osteocalcin (S-BGP) did not change. The multivariate regression analysis showed no relationship between bone density measurement and biochemical bone markers. Only the age significantly correlated negatively with bone density measurement. For a better assessment of type I collagen metabolism we created a “b-quotient” by dividing the sum of S-PICP and S-BGP by U-PYR. The median b-quotient increased significantly: I 1.55*+ (0.97–2.04); II 2.09* (1.57–2.86); III 2.46+ (1.58–3.22);*+ P〈0.05. Changes in bone metabolism cannot be identified by the determination of a single marker. However, the improved biochemical diagnostic measurement using the b-quotient may provide early information about the progression of a metabolic disorder within the interval of imaging.
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    Springer
    Journal of molecular medicine 53 (1975), S. 44-46 
    ISSN: 1432-1440
    Keywords: Aldolase ; isoenzyme ; immunochemical assay ; normal range ; acute hepatitis ; Aldolase ; Isoenzyme ; Immunchemischer Versuch ; Normwert ; akute Hepatitis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Description / Table of Contents: Zusammenfassung Die Isoenzyme der Aldolase im Serum wurden mit Hilfe präzipitierender Antikörper bestimmt. Das Isoenzymmuster bei 130 gesunden Personen, aus einem Normwertprogramm ausgewählt, ergab folgende Werte: Gesamt-Aldolase 0,8, 1,6, 2,5 U/L, Isoenzym A 0,6, 1,2, 1,9 U/L, Isoenzym B 0,0, 0,2 0,7 U/L und Isoenzym C 0,0, 0,1, 0,4 U/L ausgedrückt als $$\bar x$$ ±2s. Der Vergleich der Histogramme zeigt, daß die Werteverteilung bei Messung der Gesamtaldolase sich bei Gesunden und Hepatitis-Kranken überschneidet. Bei Messung der Aldolase B kommt es zu keiner Überschneidung der Werteverteilung. Eine Differenzierung zwischen Gesunden und Kranken mit akuter Hepatitis ist möglich.
    Notes: Summary Analysis of human serum aldolase isoenzymes A, B and C activities was performed by means of a recently developed immunochemical assay. Isoenzyme patterns were established in 130 healthy subjects selected from a normal range research program. Total aldolase activity ranged from 0.8, 1.6, 2.5 U/L, Ald A from 0.6, 1.2, 1.9 U/L, Ald B 0.0, 0.2, 0.7 U/L, Ald C 0.0, 0.1, 0.4 U/L expressed as $$\bar x$$ ±2s range. Comparing the histograms of total aldolase activity in patients with acute hepatitis and normal controls, an almost identical frequency distribution in both groups was observed. However, histograms of aldolase isoenzyme B values of these groups were practically completely separated. Thus in contrast with total aldolase activity, the determination of aldolase isoenzyme B activity is a useful criterion in the diagnosis of acute hepatitis.
    Type of Medium: Electronic Resource
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