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  • Articles: DFG German National Licenses  (11)
  • RAPD  (5)
  • Polymer and Materials Science  (3)
  • Allium  (2)
  • Rice  (2)
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  • Articles: DFG German National Licenses  (11)
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Keywords
  • 1
    Electronic Resource
    Electronic Resource
    Detection of somaclonal variation in garlic (Allium sativum L.) using RAPD and cytological analysis (1999)
    Springer
    Plant cell reports 18 (1999), S. 473-477 
    Details
    ISSN: 1432-203X
    Keywords: Key wordsAllium sativum ; Garlic ; Genetic instability ; RAPD ; Somaclonal variation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Plants were regenerated by somatic embryogenesis from long-term callus cultures derived from five garlic (Allium sativum L.) cultivars. Thirty-five of these plants were subjected to RAPD analysis. The frequency of variation was found to be cultivar dependent: approximately 1% in the two clones Solent White and California Late and around 0.35% in another three clones, Chinese, Long Keeper and Madena. Certain band changes were found in regenerants of different cultivars, suggesting the existence of a mutation-sensitive part of the garlic genome. The karyotypes of another 75 regenerants derived from the same callus cultures of three parental garlic clones were examined. Of these plants, 9.3% were found to be tetraploids, 4% aneuploid and 2.6% showed a change in the position of the secondary constriction. No association could be shown between the rate of variation for molecular and cytological characters either by comparing cultivars or examining individual regenerants.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s002990050606
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Are mapped markers more useful for assessing genetic diversity? (2000)
    Springer
    Theoretical and applied genetics 100 (2000), S. 607-613 
    Details
    ISSN: 1432-2242
    Keywords: Key words AFLP ; Biodiversity ; Genetic maps ; Genetic resources ; Rice
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  Genetic diversity within populations of organisms and species is commonly measured using molecular-marker data. It has been claimed that more reliable diversity measurements can be obtained using selected genetically mapped markers to ensure that all regions of the genome are represented in the data sets employed. However, this has not been tested. In the present study, using rice (Oryza sativa L.) as a model species, we have shown that the use of unmapped AFLP markers reveals a pattern of diversity that is very similar to that obtained using a range of other marker types and which reflects the known crossability groups within this species. In contrast, we show that use of mapped-marker data can, in some cases, result in highly misleading patterns of diversity; the results obtained are critically related to the choice ofparents used in the cross from which the mapping population was produced. For diversity analyses, we propose that it is appropriate to use unmapped markers provided that the marker-type has been shown to have a wide distribution over the genome.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s001220050080
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    Paper (German National Licenses)
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  • 3
    Electronic Resource
    Electronic Resource
    The identification of duplicate accessions within a rice germplasm collection using RAPD analysis (1995)
    Springer
    Theoretical and applied genetics 90 (1995), S. 1049-1055 
    Details
    ISSN: 1432-2242
    Keywords: Oryza sativa ; Rice ; Genetic resources ; RAPD ; Molecular markers ; Cluster analysis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract A set of accessions of Oryza sativa from the International Rice Research Institute (Philippines) that included known and suspected duplicates as well as closely related germplasm has been subjected to RAPD analysis. The number of primers, the number of polymorphic bands and the total number of bands were determined that will allow the accurate discrimination of these categories of accessions, including the identification of true and suspected duplicates. Two procedures have been described that could be employed on a more general basis for identifying duplicates in genetic resources collections, and further discussion on the values of such activities is presented.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00222920
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    Paper (German National Licenses)
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  • 4
    Electronic Resource
    Electronic Resource
    Numerical analyses of RAPD data highlight the origin of cultivated tagasaste (Chamaecytisus proliferus ssp. palmensis) in the Canary Islands (1993)
    Springer
    Theoretical and applied genetics 87 (1993), S. 264-270 
    Details
    ISSN: 1432-2242
    Keywords: Chamaecytisus ; Tagasaste ; Fodder legumes ; Multivariate analysis ; RAPD ; Plant genetic resources ; Biodiversity
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Random Amplified Polymorphic DNA (RAPD) was used to generate molecular markers to trace the origin of the fodder legume tagasaste (Chamaecytisus proliferus (L. fil.) Link ssp. palmensis (H. Christ) Kunkel) in the Canary Islands. Results from multivariate analyses of data through “Two Way Indicator Species Analysis” (TWINSPAN) and “Detrended Correspondence Analysis” (DECORANA) showed that genotypes collected on the island of La Palma exhibited a wider range of variation than those from the other islands. This supports the existing hypothesis that tagasaste originated on La Palma and emphasizes the importance of conserving and evaluating germ plasm from this island.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00223775
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    Paper (German National Licenses)
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  • 5
    Electronic Resource
    Electronic Resource
    Comparative Analysis of Crossover Exchanges and Chiasmata in Allium Cepa × Fistulosum After Genomic In Situ Hybridization (GISH) (1998)
    Springer
    Chromosome research 6 (1998), S. 567-574 
    Details
    ISSN: 1573-6849
    Keywords: Allium ; chiasmata ; crossing over ; genomic in situ hybridization ; recombination
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Genomic in situ hybridization (GISH) successfully differentiated homoeologous genomes in the interspecific hybrid Allium cepa × fistulosum, thus allowing the detection of reciprocal crossover events as label exchanges in separating anaphase I chromosomes. Three of the eight chromosome pairs were positively identified by fluorescence in situ hybridization (FISH) to rDNA sequences. There was a general similarity of the GISH-based label exchange frequencies and metaphase I chiasma frequencies, but with a 20% deficit of chiasmata. Reasons for this apparent deficit are discussed. The locations of chiasmata and label exchanges are in broad agreement.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1009296826942
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    Paper (German National Licenses)
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  • 6
    Electronic Resource
    Electronic Resource
    Distribution of a 375 bp repeat sequence inAllium (Alliaceae) as revealed by FISH (1999)
    Springer
    Plant systematics and evolution 217 (1999), S. 31-42 
    Details
    ISSN: 1615-6110
    Keywords: Alliaceae ; Allium ; Fluorescent in-situ hybridisation ; cytotaxonomy ; telomeric repeat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Information about evolutionary relationships between species of the genusAllium is desirable in order to facilitate breeding programmes. One approach is to study the distribution of repetitive DNA sequences among species thought on taxonomic grounds, to be closely related. We have used fluorescent in-situ hybridisation (FISH) to examine seven species within sect.Cepa of the genus (A. altaicum, A. cepa, A. fistulosum, A. galanthum, A. pskemense, A. oschaninii andA. vavilovii), one species from sect.Rhizirideum (A. roylei), two species from sect.Allium (A. sativum andA. porrum) and one species from sect.Schoenoprasum (A. schoenoprasum). Each species was probed using a 375 bp repeat sequence isolated fromA. cepa (Barnes & al. 1985), which was generated and labelled by polymerase chain reaction (PCR). No signals were detected in anyAllium species not belonging to sect.Cepa with the exception ofA. roylei, whose designation in sect.Rhizirideum is now questioned. Within sect.Cepa the probe was found to hybridize to the ‘terminal’ regions of the chromosome arms of all the species examined. In addition a number of interstitial bands were detected. Use of FISH reveals a more detailed map of the location of the repeat sequences than has previously been obtained by C-banding and other staining procedures. The distribution of the terminal and interstitial sites when compared, allow us to identify three species groups namely,A. altaicum andA. fistulosum; A. cepa, A. roylei, A. oschaninii andA. vavilovii; andA. galanthum andA. pskemense.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00984920
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    Paper (German National Licenses)
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  • 7
    Electronic Resource
    Electronic Resource
    The taxonomic characterisation of annual Beta germplasm in a genetic resources collection using RAPD markers (1996)
    Springer
    Euphytica 91 (1996), S. 205-212 
    Details
    ISSN: 1573-5060
    Keywords: Beta ; genetic resources ; RAPD ; annual beets ; molecular marker ; polymorphism
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Annual beets in the genus Beta section Beta represent an important genetic resource. Representative accessions of annual beets from a beet germplasm collection were analysed using RAPD to assess the patterns of variation and relationships among them. Using arbitrary primers, markers showing variation across accessions were identified. A dendrogram of similarity was produced using these molecular markers. All the accessions analysed were classified into three major groups corresponding to species or subspecies macrocarpa, adanensis and maritima. Macrocarpa was shown to be the most divergent group in this section. Using RAPD molecular markers, it was possible to ascribe an accession to one of three taxonomic groups and overcome much of the confusion encountered when morphological traits are used for identification. The group of maritima was found to be more polymorphic than either the group of macrocarpa or adanensis at both accession and subspecies levels.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00021071
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    Paper (German National Licenses)
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  • 8
    Electronic Resource
    Electronic Resource
    The use of RAPD for assessing variation in plants (1993)
    Springer
    Plant growth regulation 12 (1993), S. 43-51 
    Details
    ISSN: 1573-5087
    Keywords: RAPD ; fingerprinting ; polymorphism
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00144581
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    Paper (German National Licenses)
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  • 9
    Electronic Resource
    Electronic Resource
    Fracture behavior in nylon 6 fibers (1972)
    New York : Wiley-Blackwell
    Journal of Polymer Science Part A-2: Polymer Physics 10 (1972), S. 1415-1445 
    Details
    ISSN: 0449-2978
    Keywords: Physics ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Physics
    Notes: A reaction rate model of fracture in polymer fibers is described. This model assumes that bond rupture is governed by absolute reaction rate theory with a stress-aided activation energy. It is demonstrated that the key in obtaining good agreement between the model and experiment lies in taking proper account of the variation of stress on the tie-chain molecules. The more taut chains rupture first, and the load is redistributed among the remaining unruptured tie chains. The effect of varying the temperature both in the model and in experiments on fracture in fibers is explored. Good agreement between predictions of the model and experiment is possible only with an undeterstanding of the distribution in stress on the tie chains. The distribution in stress on the chains was experimentally determined by monitoring the kinetics of bond rupture with electron paramagnetic resonance (EPR) spectroscopy. Temperature is found to have two effects on macroscopic strength. (1) The thermal energy aids the atomic stress in breaking the atomic bonds; as a consequence the rate of bond rupture of a family of bonds under a given molecular stress is increased. In this respect temperature might be viewed as decreasing the “strength” of a bond. (2) Temperature also serves to “loosen” the molecular structure and in this way modify the distribution in stress on the tie chains. To explain bond rupture and macroscopic fracture behavior quantitatively, account must be taken of both effects.
    Additional Material: 13 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/pol.1972.160100802
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    Paper (German National Licenses)
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  • 10
    Electronic Resource
    Electronic Resource
    Crystallite orientation and spiral structure of cotton. Part II. Decrystallized and mercerized cottons (1961)
    Hoboken, NJ : Wiley-Blackwell
    Journal of Polymer Science 54 (1961), S. 471-489 
    Details
    ISSN: 0022-3832
    Keywords: Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Physics
    Notes: A method is demonstrated by which both crystallite orientation and spiral angle can be directly determined from the x-ray tracings of the 002 diffraction are of combed bundles of cotton fibers. Balls has shown that cotton has a spiral structure, and Sisson has shown qualitatively that these 002 arcs can be explained by applying two equal crystallite distributions separated by twice the spiral angle. If this distribution of crystallites is assumed to be a Gaussian distribution, the experimental are can be generated theoretically. This method of analysis is applied to x-ray data for decrystallized and mercerized cottons. X-ray diffraction shows the effects on fine structure of after-treatments, such as tension and boiling in water, applied to samples of slack amine-decrystallized and mercerized cotton yarns. The comparison of the calculated parameters with those of the gross arcs are given, as well as the relationships of these quantities to the tensioning force which produced them.
    Additional Material: 4 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/pol.1961.1205416013
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    Paper (German National Licenses)
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