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  • 1
    Electronic Resource
    Electronic Resource
    Springer
    Fresenius' Zeitschrift für analytische Chemie 261 (1972), S. 257-271 
    ISSN: 1618-2650
    Keywords: Analysatoren ; Entwicklung eines schnellen Analysators für physiologische Proben
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Description / Table of Contents: Zusammenfassung Im Oak Ridge National Laboratory wurde ein neues Analysatorsystem entwickelt, das für die Analyse physiologischer Proben bestimmt ist und in klinischen Laboratorien, Krankenhäusern, Unfallstationen, Forschungsinstituten sowie in einer verkleinerten Version auch in der Raumfahrt zum Einsatz gelangen kann. Das System zeichnet sich aus durch leichte Automatisierbarkeit, Flexibilität, rasche Inbetriebnahme und eignet sich für parallel durchgeführte Serienanalysen. Die folgenden Charakteristica des Systems werden diskutiert: 1. Dynamisches Mischen von Proben und Reagentien durch zentrifugale Übertragung in die Küvetten, 2. Entleeren, Spülen und Trocknen der Küvetten durch Rotation, 3. Genaue Temperaturkontrolle, 4. Spektrophotometrische Reaktionsüberwachung, 5. Datenberechnung mit Speicherung aller Werte für möglichen Abruf, 6. Feedback-Kontrolle zur Fehlerkorrektur und Zeiteinsparung. Mögliche zukünftige Verbesserungen werden erwähnt.
    Notes: Summary New instrumentation has been developed at ORNL for analyzing physiologic specimens. The system is designed for use in clinical laboratories, hospitals, and emergency rooms and for use by researchers. Miniaturized versions may also be used in prolonged space flights. After consideration of ease of automation, flexibility, and rapid startup, multiple analyses performed in parallel was chosen as the desired approach. Concepts employed in developing the system are discussed, including: (1) dynamic mixing of samples and reagents through centrifugal transfer from transfer disks into cuvets; (2) rotor design allowing emptying, flushing, and drying of cuvets; (3) precise temperature control; (4) spectrophotometric reaction monitoring; (5) data reduction, with all data stored for possible recall; (6) feedback control for error correction and time saving. Possible future improvements are indicated.
    Type of Medium: Electronic Resource
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  • 2
    ISSN: 0173-0835
    Keywords: Two-dimensional polyacrylamide gel electrophoresis ; Liver ; Map ; Identification ; Calibration ; Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: We have improved upon the reference two-dimensional (2-D) electrophoretic map of rat liver proteins originally published in 1991 (N. L. Anderson et al., Electrophoresis 1991, 12, 907-930). A total of 53 proteins (102 spots) are now identified, many by microsequencing. In most cases, spots cut from wet, Coomassie Blue stained 2-D gels were submitted to internal tryptic digestion [2], and individual peptides, separated by high-performance liquid chromatography (HPLC), were sequenced using a Perkin-Elmer 477A sequenator. Additional spots were identified using specific antibodies.
    Additional Material: 3 Ill.
    Type of Medium: Electronic Resource
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