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  • Articles: DFG German National Licenses  (10)
  • Chemical Engineering  (5)
  • Analytical Chemistry and Spectroscopy  (3)
  • Binding site (ethylene)  (2)
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  • Articles: DFG German National Licenses  (10)
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  • 1
    Electronic Resource
    Electronic Resource
    The effect of solubilisation on the character of an ethylene-binding site from Phaseolus vulgaris L. cotyledons (1984)
    Springer
    Planta 160 (1984), S. 474-479 
    Details
    ISSN: 1432-2048
    Keywords: Binding site (ethylene) ; Ethylene (binding site) ; Phaseolus (ethylene binding)
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The ethylene-binding site (EBS) from Phaseolus vulgaris cv. Canadian Wonder cotyledons can be solubilised from 96,000 g pelleted material by Triton X-100 or sodium cholate. Extraction of 96,000 g pellets with acetone, butanol or butanol and ether results in a total loss of ethylene-binding activity. Like the membrane-bound form, the solubilised EBS has an apparent KD(liquid) of 10-10 M at a concentration of 32 pmol EBS per gram tissue fresh weight. Propylene and acetylene act as competitive inhibitors, carbon dioxide appears to promote ethylene binding and ethane has no significant effect. The solubilised EBS is completely denatured affect. The solubilised EBS is completely denatured after 10 min at 70°C, by 1 mM mercaptoethanol and 0.1 mM dithiothreitol, but not by trypsin or chymotrypsin. However, solubilisation decreases the rate constant of association from 103 M-1 s-1 to 101–102 M-1 s-1 and hence does not permit experimental determination of the rate constant of dissociation. The pH optimum for ethylene binding is altered from the range pH 7–10 in the membrane-bound form to the pH range 4–7 in the solubilised form. The EBS appears to be a hydrophobic, intergral membrane protein, which requires a hydrophobic environment to retain its activity. Partitioning of the EBS into polymer phases is determined by the detergent used for solubilisation indicating that when solubilised, the EBS forms a complex with detergent molecules.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00429766
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Partial purification of an ethylene-binding site from Phaseolus vulgaris L. cotyledons (1985)
    Springer
    Planta 164 (1985), S. 272-277 
    Details
    ISSN: 1432-2048
    Keywords: Binding site (ethylene) ; Ethylene (binding site) ; Phaseolus (ethylene binding)
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The solubilised ethylene-binding site (EBS) of Phaseolus vulgaris L. cotyledons is an asymmetrical protein with a sedimentation coefficient of 2 S and a Stoke's radius of 6.1 nm (determined by ultracentrifugation on isokinetic gradients and gel-permeation chromatography, respectively). The molecular weight and frictional ratio were calculated as 52 000–60 000 and 2.37–2.48, respectively. The EBS has an isoelectric point at between pH 3–5, determined by isoelectric focussing and exhibits a negative charge at pH 8 during non-denaturing electrophoresis. The electrical charge on the EBS is shielded; the EBS does not bind to anion-exchange media under the experimental conditions reported here, is not precipitated by ammonium sulphate and does not precipitate at its isoelectric pH. The EBS preferentially partitions into detergent phases. The results indicate that the EBS is a hydrophobic protein complexed with detergent in aqueous solution. The techniques used to characterise the EBS also resulted in varying degress of purification.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00396092
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    Paper (German National Licenses)
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  • 3
    Electronic Resource
    Electronic Resource
    A 2-d numerical study of intermeshing self-wiping screws in biopolymer extrusion (1991)
    Stamford, Conn. [u.a.] : Wiley-Blackwell
    Polymer Engineering and Science 31 (1991), S. 1157-1163 
    Details
    ISSN: 0032-3888
    Keywords: Chemistry ; Chemical Engineering
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics , Physics
    Notes: A knowledge of flow behavior is important in the study of laminar flow in twin-screw extrusion processes to predict the velocity distribution and to understand the mixing process. The flow of a power law fluid in self-wiping twin-screw extruders is examined using a two-dimensional finite element analysis of a mid-channel section of intermeshing screws. Theory is compared with experiment using food biopolymer and plastic materials. Comparisons showing overprediction of throughputs, but similarities in behavior, suggest that this model could provide an upper limit for melt conveying. For most of the throughput range examined, pumping of intermeshing self-wiping screws appears to be almost independent of the power law flow index of the melt extruded, but the value of the flow index determines the degree of influence intermeshing has on the overall pressure gradient generated in the extruder.
    Additional Material: 6 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/pen.760311513
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    Paper (German National Licenses)
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  • 4
    Electronic Resource
    Electronic Resource
    Family plots for evaluating physical properties of organosilicon compounds (1994)
    Hoboken, NJ : Wiley-Blackwell
    AIChE Journal 40 (1994), S. 373-377 
    Details
    ISSN: 0001-1541
    Keywords: Chemistry ; Chemical Engineering
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Process Engineering, Biotechnology, Nutrition Technology
    Additional Material: 6 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/aic.690400217
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    Paper (German National Licenses)
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  • 5
    Electronic Resource
    Electronic Resource
    Stable isotope ratio analysis of leucine and ketoisocaproic acid in blood plasma by gas chromatography/mass spectrometry. Use of tertiary butyldimethylsilyl derivatives (1988)
    New York, NY : Wiley-Blackwell
    Rapid Communications in Mass Spectrometry 2 (1988), S. 14-16 
    Details
    ISSN: 0951-4198
    Keywords: Chemistry ; Analytical Chemistry and Spectroscopy
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Physics
    Additional Material: 3 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/rcm.1290020105
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    Paper (German National Licenses)
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  • 6
    Electronic Resource
    Electronic Resource
    Pressure losses in the packing stage of injection molding (1986)
    Stamford, Conn. [u.a.] : Wiley-Blackwell
    Polymer Engineering and Science 26 (1986), S. 1282-1289 
    Details
    ISSN: 0032-3888
    Keywords: Chemistry ; Chemical Engineering
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics , Physics
    Notes: The pressure loss between the mold and the nozzle in the injection molding of bar and box moldings has been monitored. The pressure drop observed during filling of the mold is reduced during the packing stage but remains finite. This has been attributed in the literature to solidification of polymer across the cavity transducer and to melt relaxation phenomena. Experiments have been carried out with hot molds to prolong the packing stage at the expense of the ‘cooling’ stage. Under these circumstances the pressure drop is reduced but not eliminated. The observed pressure drop may be related to the viscosity of the melt and its dependence on pressure and temperature although strain-induced crystallization and the pressure dependence of the melting point can confer effects similar to the cooling stage.
    Additional Material: 9 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/pen.760261808
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    Paper (German National Licenses)
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  • 7
    Electronic Resource
    Electronic Resource
    Pumping characteristics of self-wiping twin-screw extruders - A theoretical and experimental study on biopolymer extrusion (1989)
    Stamford, Conn. [u.a.] : Wiley-Blackwell
    Polymer Engineering and Science 29 (1989), S. 433-440 
    Details
    ISSN: 0032-3888
    Keywords: Chemistry ; Chemical Engineering
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics , Physics
    Notes: The flow of Newtonian and non-Newtonian fluids which obeys a power law relationship between shear stress and shear rate has been modeled in the melt conveying section of a self-wiping co-rotating twin-screw extruder using a finite element analysis of an unwound channel section. Predictions of throughput against pressure gradient are compared with experimentally obtained results for maize grits which is represented as a power law material. Rheological data applicable to extrusion simulation were obtained from capillary rheometry. Comparisons are reasonable with predicted characteristic showing similar behavior.
    Additional Material: 6 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/pen.760290702
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    Paper (German National Licenses)
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  • 8
    Electronic Resource
    Electronic Resource
    Some features of the injection molding of short fiber reinforced thermoplastics in center sprue-gated cavities (1987)
    Brookfield, Conn. : Wiley-Blackwell
    Polymer Composites 8 (1987), S. 16-21 
    Details
    ISSN: 0272-8397
    Keywords: Chemistry ; Chemical Engineering
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
    Notes: This paper describes an investigation into the fiber orientation in a number of center sprue fed cavities in short glass fiber filled polypropylene and nylon. The data have been interpreted in terms of a generalized five-layer structure resulting from the frozen skin formation and the high and low shear levels in the flowing melt. The implications for scaling up the mold size are discussed from the results obtained with different shot volumes. The fiber structure was observed to depend on location in the molding, local injection time, and injection rate. In addition the occurrence of fiber-free layers within the moldings using the filled polypropylene increases with an increase in shot volume, which produces an inherent ‘scale-up’ problem. Notwithstanding the mold geometry subtleties, the fiber orientation in all the moldings follows similar patterns and trends.
    Additional Material: 8 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/pc.750080104
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    Paper (German National Licenses)
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  • 9
    Electronic Resource
    Electronic Resource
    Identification of branched chain fatty acids in baboon liver lipids (1979)
    Chichester : Wiley-Blackwell
    Biological Mass Spectrometry 6 (1979), S. 345-346 
    Details
    ISSN: 0306-042X
    Keywords: Chemistry ; Analytical Chemistry and Spectroscopy
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: A total of 26 monomethyl branched saturated fatty acids was identified in baboon liver lipids; these included a novel anteiso component with an odd number of carbon atoms in the chain (13-methylpentadecanoic acid).
    Additional Material: 1 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/bms.1200060807
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    Paper (German National Licenses)
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  • 10
    Electronic Resource
    Electronic Resource
    Characterization of ethyl substituted fatty acids from lamb subcutaneous triacylglycerols by combined gas chromatography mass spectrometry (1979)
    Chichester : Wiley-Blackwell
    Biological Mass Spectrometry 6 (1979), S. 347-349 
    Details
    ISSN: 0306-042X
    Keywords: Chemistry ; Analytical Chemistry and Spectroscopy
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: Mid-chain ethyl substituted fatty acids with chain lengths ranging from 10 to 16 carbon atoms were identified in a concentrate of branched chain fatty acids isolated from the triacylglycerol fatty acids of the subcutaneous adipose tissue of barley-fed lambs. The acids were identified by gas chromatography in combination with mass spectrometry and their mass spectral features are discussed.
    Additional Material: 2 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/bms.1200060808
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