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  • Articles: DFG German National Licenses  (6)
  • Analytical Chemistry and Spectroscopy  (2)
  • fatty acids  (2)
  • Ca2+ pump  (1)
  • Column liquid chromatography  (1)
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  • Articles: DFG German National Licenses  (6)
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  • 1
    Electronic Resource
    Electronic Resource
    Effect of hydralazine on myocardial plasma membrane fatty acid binding protein (PM-FABP) during diabetes mellitus (1995)
    Springer
    Molecular and cellular biochemistry 148 (1995), S. 39-44 
    Details
    ISSN: 1573-4919
    Keywords: diabetes mellitus ; cardiomyopathy ; hydralazine ; cardiomyocytes ; fatty acids ; fluorescence ; trans-parinaric acid ; cis-parinaric acid ; plasma membrane fatty acid binding protein
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology , Medicine
    Notes: Abstract Chronic treatment with the antihypertensive drug hydralazine did not affect the hyperglycemic state of streptozotocin (STZ)-diabetic rats but did prevent the serum hyperlipidemia that is synonymous with these diabetic animals. After 6 weeks, untreated STZ-diabetic rats exhibited a 659% increase in serum triglycerides and 292% increase in serum cholesterol versus age-matched non-diabetic rats. Hydralazine-treated STZ-diabetic rats had serum triglyceride and cholesterol levels that did not differ from controls. Myocytes from control rats showed a preference for binding of the unsaturated fatty acid analog cis-parinaric acid vs the saturated fatty acid analog trans-parinaric acid. This preference was not altered in STZ-diabetic rat myocytes; hydralazine-treatment of STZ-diabetic rats also showed no change in fatty acid preference. STZ-diabetes caused a decrease in the affinity (Kd) for the trans, but not the cis-parinaric acid. However, total binding of both analogs was increased in STZ-diabetes. Hydralazine treatment of STZ-diabetic rats resulted in even greater total binding of both analogs. Affinity for the trans analog was further decreased in these hydralazine-treated rats, but the affinity for the cis analog was increased beyond control animals. These results suggest that the diabetic state influences the binding characteristics of the myocardial PM-FABP and that hydralazine, while reducing serum lipids in diabetes, has complex effects on the fatty acid binding by this protein.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00929501
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    Paper (German National Licenses)
    Fulltext
  • 2
    Electronic Resource
    Electronic Resource
    Effect of calmodulin on sarcoplasmic reticular Ca2+-transport in the aging heart (1989)
    Springer
    Molecular and cellular biochemistry 85 (1989), S. 75-791 
    Details
    ISSN: 1573-4919
    Keywords: aging myocardium ; Ca2+ pump ; sarcoplasmic reticulum ; calmodulin ; Ca2+ uptake
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology , Medicine
    Notes: Abstract Heart failure is common among the elderly and an alteration in myocardial Ca2+ transport is believed to be involved in its depressed contractile performance. Although ATP-dependent sarcoplasmic reticular (SR) Ca2+ transport has been reported to decrease in old hearts, virtually nothing appears to be known about the Ca2+ pump activity of SR in aging myocardium in the presence of calmodulin, one of its endogenous activators. In this study, the activity of the Ca2+ pump of aging cardiac SR was assessed in the presence of this endogenous stimulator. This assessment was therefore designed to give additional information about the status of this enzyme in old hearts. Male Sprague-Dawley rats were used and were divided into 3 groups: young (4–6 months old); middle-aged (15–17 months old) and old age (24–25 months old). Purified SR membranes were isolated from ventricular tissues. ATP-dependent Ca2+ accumulation by membrane vesicles of middle-aged and old hearts was significantly depressed in comparison to young hearts at all Ca2+ concentrations employed in the absence and presence of calmodulin. The activity of this Ca2+ transporter was similar in middle-aged and old hearts even in the presence of calmodulin. These results suggest that the activity of the Ca2+ pump in SR of aging hearts is depressed even in the presence of calmodulin.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00223516
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    Paper (German National Licenses)
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  • 3
    Electronic Resource
    Electronic Resource
    Characteristics of the myocardial PM-FABP: Effect of diabetes mellitus (1997)
    Springer
    Molecular and cellular biochemistry 176 (1997), S. 281-286 
    Details
    ISSN: 1573-4919
    Keywords: diabetes mellitus ; Diabetic cardiomyopathy ; fatty acids ; fatty acid binding protein
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology , Medicine
    Notes: Abstract Properties of the myocardial PM-FABP were studied in normal and STZ-diabetic rats. The fluorescent fatty acids trans-parinaric and cis-parinaric acids were used as analogs of straight-chain (saturated) and kinked-chain (unsaturated) fatty acids respectively. Parinaric acid binding was sensitive to trypsin. Trans-parinaric acid binding was more sensitive to this protease than the binding of cis-parinaric acid. Based on the difference in sensitivity of parinaric acid binding we believe that there are two separate binding sites associated with myocardial PM-FABP; one for unsaturated fats and the other for saturated fats. Diabetes enhanced both cis- and trans-parinaric acid binding capacity in cardiomyocytes; cis-parinaric acid by 2 fold and trans-parinaric acid by 2.6 fold. In addition, there was a concomitant accumulation of free fatty acids and triglycerides in the hearts of the diabetic animals. There was a 2.2 fold increase for fatty acids and a 1.6 fold increase for trigylcerides. This association between myocardial fatty acid build-up and enhanced myocardial PM-FABP during diabetes suggest that this carrier protein might have contributed to lipid accumulation in the hearts of the diabetic rats.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1006817632484
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    Paper (German National Licenses)
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  • 4
    Electronic Resource
    Electronic Resource
    The application of high performance liquid chromatography, coupled to nuclear magnetic resonance spectroscopy and mass spectrometry (HPLC-NMR-MS), to the characterisation of ibuprofen metabolites from human urine (1998)
    Springer
    Chromatographia 47 (1998), S. 264-270 
    Details
    ISSN: 1612-1112
    Keywords: Column liquid chromatography ; NMR and MS detection ; Ibuprofen metabolites ; Urine extracts
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Summary The use of HPLC-NMR-MS for the detection and identification of the metabolites of ibuprofen present in a solid phase extract of human urine is described. Gradient reversed-phase HPLC was used to separate the components present in the extract, which were then characterised by a combination of stopped-flow1H NMR and on line electrospray-MS. This approach led to the rapid identification of the known phase 1 human metabolites of ibuprofen, including hydroxy- and carboxy- metabolites, together with their respective glucuronide conjugates. In addition a probable artefact resulting from the dehydration of one of the side chainhydroxylated glucuronides was also identified.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02466530
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    Paper (German National Licenses)
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  • 5
    Electronic Resource
    Electronic Resource
    A combined magnetic sector-time-of-flight mass spectrometer for structural determination studies by tandem mass spectrometry (1995)
    New York, NY : Wiley-Blackwell
    Rapid Communications in Mass Spectrometry 9 (1995), S. 1227-1233 
    Details
    ISSN: 0951-4198
    Keywords: Chemistry ; Analytical Chemistry and Spectroscopy
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Physics
    Notes: A combined magnetic sector-time-of-flight instrument has been constructed and used with both continuous (liquid secondary ion mass spectrometry and electrospray ionization) and pulsed (matrix-assisted laser desorption/ionization) ion sources. The design includes provision for both low- and high-energy gas collision induced decompositions and orthogonal acceleration of the product ions into the time-of-flight mass analyser. Tandem mass spectra may be acquired for singly and multiply charged precursor ions and reliable mass measurements are easily obtained. Product-ion mass spectra are quite reproducible regardless of any instability in the precursor ion beam intensity.
    Additional Material: 12 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/rcm.1290091302
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    Paper (German National Licenses)
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  • 6
    Electronic Resource
    Electronic Resource
    Letter to the editor (1992)
    New York, NY : Wiley-Blackwell
    Rapid Communications in Mass Spectrometry 6 (1992), S. 719-720 
    Details
    ISSN: 0951-4198
    Keywords: Chemistry ; Analytical Chemistry and Spectroscopy
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Physics
    Additional Material: 1 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/rcm.1290061118
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    Paper (German National Licenses)
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