ISSN:
1617-4623
Keywords:
Bkm DNA
;
Genetic/DNA fingerprinting
;
Crocodilians
;
Paternity determination
;
Band sharing
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract Individual-specific DNA fingerprints of crocodilians were obtained by the use of Bkm-2(8) probe. Pedigree analyses of Crocodylus palustris, C. porosus and Caiman crocodilus revealed that the multiple bands (22–23 bands with Aludigest) thus obtained were inherited stably in a Mendelian fashion. Unique fingerprints permitted us to identify individuals, assign parentage, and reconstruct the DNA profile of a missing parent. Average band sharing between unrelated crocodiles was found to be 0.37. Band sharing between animals of known pedigrees increased predictably with relatedness and provided a basis for distinguishing relatives from non-relatives. Similar results obtained in other species/genera, using the same probe, suggest that this approach may be applicable to all species of crocodilians, and could facilitate genetic studies of wild and captive populations.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00279530
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