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  • Articles: DFG German National Licenses  (2)
  • Apoptosis  (1)
  • Baetis spp.  (1)
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  • Articles: DFG German National Licenses  (2)
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Years
  • 1
    ISSN: 1432-1262
    Keywords: Keywords Nonsteroidal anti-inflammatory drugs ; Colon cancer ; Apoptosis ; Caspase ; Poly(ADP-ribose) polymerase
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract  Epidemiological studies have demonstrated that nonsteroidal anti-inflammatory drugs (NSAIDs) decrease the incidence of and mortality from colon cancer. In addition, NSAIDs reduce the number and the size of polyps in patients with familial adenomatous polyposis. The mechanisms responsible for the antineoplastic effect of NSAIDs are not yet completely understood, but one of the possible mechanisms is an induction of apoptosis. We explored the role of caspase-3, a major apoptosis-executing enzyme, in NSAID-induced apoptosis of colon cancer cell line HT-29. Treatment of HT-29 cells with indomethacin induced a dramatic increase in caspase-3-like protease activity measured by a cleavage of the fluorogenic substrate Ac-DEVD-AMC. Western blot analysis showed that indomethacin treatment led both to decrease in pro-caspase-3 and to cleavage of its substrate poly(ADP-ribose) polymerase (PARP). Furthermore, the caspase- 3-like protease inhibitor Ac-DEVD-CHO attenuated indomethacin- induced DNA fragmentation dose dependently. However, mRNA expression of CASP genes was not affected by the addition of indomethacin, highlighting the importance of posttranslational modification of this enzyme for the activation. These results suggest that NSAIDs, including indomethacin, induce apoptosis in colon cancer cells through a caspase-3 dependent mechanism which may contribute to the chemopreventive functions of these agents.
    Type of Medium: Electronic Resource
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  • 2
    ISSN: 1573-5117
    Keywords: acid deposition ; stream channels ; drift ; benthos ; Baetis spp. ; Sierra Nevada
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The effects of pulsed acidification on invertebrate densities and drift, and water chemistry, in a high altitude Sierra Nevada stream were measured using artificial stream channels. Water was diverted from the Marble Fork of the Kaweah River, California, U.S.A., through twelve replicate channels; however, low flow in the summer of 1985 eliminated all but four of these channels. Channels were stocked with natural substrates and organisms from the Marble Fork of the Kaweah River. After a three week acclimation period, we simulated a low pH rain event by adding acid (H2SO4 and HNO3) to two of the channels, reducing pH to 5.0 for 6 hours. The other two channels acted as controls (pH 6.4). During acid additions, Baetis spp. drift in acidified channels was ca. 7 times higher than in control channels (F = 39.02, p 〈 0.025; data fourth root transformed, ANOVA), and the percentage of drifting baetids that was dead was significantly higher in acidified than control channels (46% vs. 0%, F = 29.86, p 〈 0.05; arcsine square root transformed data, ANOVA). Other taxa showed no significant drift responses, and benthic densities of all taxa showed no effects two days after acidification, probably owing to rapid recolonization by invertebrate drift in influent waters. Stream chemistry data are presented; heavy metal concentrations did not significantly increase in the 2 m stream channels.
    Type of Medium: Electronic Resource
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